槲皮素对肉牛体外瘤胃发酵及甲烷产量的影响  

Effects of quercetin on in vitro rumen fermentation and methane production of beef cattle

作　　者：杜柳 魏曼琳[1] 巨纪 董晨扬 张润泽 晓明 孙芳[2] DU Liu;WEI Manlin;JU Ji;DONG Chenyang;ZHANG Runze;XIAO Ming;SUN Fang(College of Animal Science and Technology,Inner Mongolia University for Nationalities,Tongliao 028000,Inner Mongolia,China;Institute of Animal Husbandry,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Heilongjiang,China)

机构地区：[1]内蒙古民族大学动物科技学院,内蒙古通辽028000 [2]黑龙江省农业科学院畜牧研究所,黑龙江哈尔滨150086

出　　处：《草业科学》2023年第1期280-286,共7页Pratacultural Science

基　　金：农业农村部“农牧交错带牛羊牧繁农育关键技术集成示范项目”(16190050、16200158、16210096);黑龙江省农业科学院“农业科技创新跨越工程”专项(2019CX-16);内蒙古民族大学研究生科研创新项目“饲粮中添加槲皮素对肉牛瘤胃体外发酵、甲烷产生和微生物区系的影响”(NMDSS2138)。

摘　　要：为研究饲粮中添加槲皮素对肉牛瘤胃体外发酵特性及甲烷产量的影响,采用单因子试验设计,分别在体外发酵底物中添加0、0.5%和1%的槲皮素,以不添加槲皮素为对照组,在39℃下进行瘤胃厌氧培养发酵,记录不同时间的产气量(GP),测定48 h的发酵液pH、氨态氮(NH3-N)浓度、挥发性脂肪酸浓度(VFA)和甲烷产量,同时,利用体外两步法测定发酵48 h后的干物质降解率(DMD)。结果显示:1) 48 h后,1%槲皮素组产气量、甲烷产量显著低于对照组(P <0.05);各组间pH无显著差异(P> 0.05);0.5%和1%槲皮素组NH3-N均显著低于对照组(P <0.05)。2)槲皮素对干物质降解率无显著影响(P> 0.05);0.5%和1%槲皮素组的乙酸、丁酸和总VFA均无显著差异(P> 0.05),1%槲皮素组丙酸显著高于对照组(P <0.05)。因此,肉牛饲粮中添加槲皮素会降低肉牛瘤胃体外发酵产气量、降低NH3-N的含量、提高丙酸的含量,降低甲烷的产生量,其中以添加1%的槲皮素较为适宜。This study aimed to observe the effects of quercetin supplementation on in vitro rumen fermentation and methane production of beef cattle. A single-factor experimental design was used in this study, and the treatment groups included 0、0.5%, and 1% quercetin added to the substrate for in vitro fermentation, with no quercetin added used representing the control group. Rumen anaerobic fermentation was conducted at 39 ℃, and gas production(GP) at different times was recorded. The pH, ammonia nitrogen(NH3-N) concentration, volatile fatty acid concentration(VFA), and methane production were determined after 48 h of fermentation. In addition, the dry matter disappearance(DMD) rate after 48 h of in vitro fermentation was determined by a two-step method. The following results were obtained: 1) After 48 h, gas and methane production of the 1% quercetin group was significantly lower than that of the control group(P < 0.05);pH did not show significant differences among the groups(P > 0.05), and the NH3-N content in the 0.5% and 1% quercetin groups was significantly lower than that in the control group(P < 0.05). 2) the DMD rate was not significantly affected by quercetin(P >0.05);acetic acid, butyric acid, and total volatile fatty acids did not show significant differences between the 0.5% and 1%quercetin groups(P > 0.05), while propionic acid in 1% quercetin group was significantly higher than that in the control group(P < 0.05). In conclusion, the addition of quercetin to beef cattle diets may reduce gas production, decrease NH3-N contents, increase propionic acid contents, and decrease methane production by beef cattle, and 1% quercetin supplementation in beef cattle diets is most suitable.

关 键 词：槲皮素 体外发酵 发酵特性 氨态氮 挥发性脂肪酸 干物质降解率 甲烷产量 

分 类 号：S823.5[农业科学—畜牧学]