锯谷盗线粒体基因组及扁甲总科系统发育分析  

The mitochondrial genome of Oryzaephilus surinamensis and a phylogenetic analysis of Cucujoidea

作　　者：林兴雨 翟卿[1] 宋南[1] 尹新明[1] LIN Xingyu;ZHAI Qing;SONG Nan;YIN Xinming(Plant Protection College of Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南农业大学植物保护学院,河南郑州450002

出　　处：《河南农业大学学报》2023年第1期109-117,共9页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(U1904104);国家现代农业体系研究项目(CARS-27);调控棉铃虫信息素生物合成的研究项目(224200510018);西藏入侵害虫风险评估与重要资源昆虫保护利用研究项目(XZ202001YD0002C)。

摘　　要：【目的】探究锯谷盗Oryzaephilus surinamensis的线粒体基因组结构特征及扁甲总科的系统发育关系。【方法】利用下一代测序方法获得了锯谷盗线粒体基因组的全序列,并基于扁甲总科65个物种(内群)和2个象甲总科物种(外群)的13个蛋白质编码基因、通过最大似然法和邻接法构建扁甲总科的系统发育树。【结果】锯谷盗的线粒体基因组包含37个基因(13个蛋白质编码基因、22个tRNA基因和2个rRNA基因)和一段控制区。整个线粒体基因组全长为15711 bp(GenBank登录号:OM215199)。锯谷盗线粒体基因组的3个蛋白质编码基因(cox1,nad2和nad1)的起始密码子是ATC或GTG,其余10个蛋白质编码基因都是以ATT或ATG开头;cox1,cox2,nad2,cox3和nad3以不完整的终止密码子T结尾,其余蛋白质编码基因以完整的终止密码子TAA或TAG结尾。除trnS1因缺少DHU臂而形成一个简单的环,无法形成稳定的三叶草结构外,其余tRNA基因均能形成典型的三叶草结构。此外,trnS1的反密码子不是常见的GCU,而是UCU。rrnL基因的全长为1281 bp,A+T含量为76.58%。rrnS基因的全长为757 bp,A+T含量为75.90%。【结论】2种不同的系统发育分析方法构建的扁甲总科的系统发育关系是基本一致的,均表明扁甲科为单系群;锯谷盗科,大蕈甲科和露尾甲科均为非单系群;锯谷盗O.surinamensis与单齿谷盗Silvanus unidentatus构成姐妹群。【Objective】To explore the characteristics of the mitochondrial genome of Oryzaephilus surinamensis and to investigate the phylogenetic relationship of Cucujoidea.【Methods】In this study,we newly sequenced the complete mitochondrial genome of O.surinamensis by using next-generation sequencing method,and constructed the phylogenetic trees using Maximum Likelihood and Neighbor-Joining inference methods,based on 13 protein-coding genes of 65 Cucujoidea species(ingroup)and 2 Curculionoidea species(outgroup).【Results】The mitochondrial genome of O.surinamensis is a circular molecule of 15711 bp in length(Genbank accession no.OM215199)which possesses 37 genes(13 protein-coding genes,22 transfer RNA genes,and 2 ribosomal RNA genes)and a putative control region.Most of protein-coding genes start with the codons of ATT or ATG except for the cox1,cox2,nad2,cox3 and nad3 genes,which use ATC or GTG as start codons,respectively.Most of protein-coding genes terminate with the stop codons of TAA or TAG,while the cox1,cox2,nad2,cox3 and nad3 genes employ the incomplete T as the stop codons.All tRNA genes can be folded into typical cloverleaf structure,with the except of the trnS1.The trnS1 only forms a simple ring due to the lack of DHU arm.In addition,the anticodon of trnS1 is not the common GCU,but the UCU.The lengths of the rrnL and rrnS genes were 1281 bp and 757 bp,with the A+T contents of 76.58%and 75.90%,respectively.【Conclusion】Both phylogenetic inference methods showed the largely identical tree topological structures:the family Cucujidae is a monophyletic group,while the Silvanidae,Erotylidae and Nitidulidae are each non-monophyletic;The newly sequenced O.surinamensis is sister to Silvanus unidentatus.

关 键 词：鞘翅目 锯谷盗科 锯谷盗 线粒体基因组 系统发育 

分 类 号：S433[农业科学—农业昆虫与害虫防治]