不同经营方式的云冷杉针阔混交林土壤真菌群落结构  

Soil Fungal Community Structure under Different Management Practices in Mixed Spruce-needle Forests

作　　者：刘志宇 张忠辉[1] 杨凯麟 张军 姜润华 吴则甫 王琦 李文华 夏富才[2] Liu Zhiyu;Zhang Zhonghui;Yang Kailin;Zhang Jun;Jiang Runhua;Wu Zefu;Wang Qi;Li Wenhua;Xia Fucai(Jilin Provincial Academy of Forestry Sciences,Changchun 130033,P.R.China;Beihua University)

机构地区：[1]吉林省林业科学研究院,长春130033 [2]北华大学

出　　处：《东北林业大学学报》2023年第3期124-129,共6页Journal of Northeast Forestry University

基　　金：国家重点研发计划(2017YFC05041102)。

摘　　要：为评估不同经营方式的云冷杉林土壤真菌群落结构,以吉林省汪清林业局云冷杉针阔混交林为研究对象,设置4种经营方式,传统经营——进行抚育采伐,采伐后郁闭度在0.7以上;目标树经营1——按目标树单株经营进行采伐,保留用材目标树密度100株/hm^(2);目标树经营2——按目标树单株经营进行采伐,保留用材目标树密度150株/hm^(2);未经采伐处理的样地作为对照。通过高通量测序技术和土壤理化性质分析,比较不同经营方式的土壤真菌群落结构的差异。结果表明:(1)不同经营方式的土壤理化性质差异显著(p<0.05)。(2)采伐增加了云冷杉针阔混交林真菌共有OTU数目和特征OTU数目。(3)α多样性分析表明目标树经营1的真菌群落最为丰富,传统经营的真菌群落多样性最高。(4)β多样性分析表明不同经营措施间的差异显著高于组内差异,说明采伐强度改变了真菌群落的物种组成。土壤真菌的优势菌群对干扰的适应程度不同,采伐导致土壤质量下降,经过采伐后土壤真菌的组成更加趋向于营腐生菌群;土壤真菌群落结构在不同经营方式下表现出不同的相似性,与对照经营相比采伐改变了真菌的群落结构,说明采伐后经过短时间的恢复,土壤真菌未趋于稳定,因此,采伐可以短时间内影响土壤真菌,并且采伐有利于土壤真菌群落进行养分循环;从真菌丰度、群落结构、相似度及土壤理化性质综合分析,目标树经营1更接近对照经营,所以目标树经营1优于其他经营方式。To assess the effects of different management practices in spruce-fir forests,the Spruce-fir mixed forest in Wangqing Forestry Bureau of Jilin Province was taken as the research object,and four management methods were set up.The traditional management:the canopy density was above 0.7 after felling.Target tree management 1:felling was carried out according to target tree single plant management,and the target tree density of timber was reserved to 100 ind·ha^(-1).Target tree management 2:felling was carried out according to target tree single plant management,and the target tree density of timber was kept at 150 ind·ha^(-1).The 3 uncut pairs are also denoted as CK.Through high-throughput sequencing technology and soil physicochemical analysis,the differences of soil bacterial community structure under different management methods were compared.The results showed that:(1)There were significant differences in soil chemistry between management practices(p<0.05).(2)Logging increased the number of OTU and OTU of fungi in Spruce-fir mixed forest.(3)αdiversity analysis showed that target tree management 1 had the most abundant fungal community,while traditional tree management had the highest fungal community diversity.(4)βdiversity analysis showed that the difference between different management methods was significantly higher than that between groups,suggesting that logging changed the species composition of fungal community.Therefore,the dominant microbial community of soil fungi had different responses to disturbance,and the soil quality decreased due to logging.After logging,the composition of soil fungi tended to be supported by saprophytic bacteria.Soil fungal community structure showed different similarities under different management methods,and harvesting changed the fungal community structure compared to the control operation,indicating that the soil fungi did not stabilize after a short period of recovery after harvesting.In conclusion,harvesting can affect soil fungi for a short period of time,and it fa

关 键 词：土壤微生物 真菌 云冷杉混交林 森林经营 

分 类 号：S753.71[农业科学—森林经理学]