基于适配体识别-纳米金比色法快速检测苏丹红  

Colorimetric detection of Sudan based on the aptamer and gold nanoparticles

作　　者：黄巧 刘成江[2] 兰国伟 闫圣坤[4] 王双慧 冯晓汀 卢春霞 HUANG Qiao;LIU Chengjiang;LAN Guowei;YAN Shengkun;Wang Shuanghui;FENG Xiaoting;LU Chunxia(School of Advanced Agriculture and Bioengineering,Yangtze Normal University,Chongqing 408100,China;Institute of Agricultural Products Processing,Xinjiang Academy of Agriculture and Reclamation Science,Shihezi 832000,China;School of Chemistry and Chemical Engineering,Shihezi University,Shihezi 832000,China;Institute of Agricultural Mechanization,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)

机构地区：[1]长江师范学院现代农业与生物工程学院,重庆408100 [2]新疆农垦科学院农产品加工研究所,石河子832000 [3]石河子大学化学化工学院,石河子832000 [4]新疆农业科学院农业机械化研究所,乌鲁木齐830091

出　　处：《分析试验室》2023年第2期228-234,共7页Chinese Journal of Analysis Laboratory

基　　金：长江师范学院科研资助项目和大学生创新创业训练计划项目(202210647002)资助。

摘　　要：以苏丹红Ⅲ核酸适配体为识别元件,以无标记的纳米金为颜色指示剂,NaCl溶液作为纳米金聚集诱导剂,构建了一种简单、快速、灵敏的苏丹红可视化比色检测方法。对适配体浓度、NaCl浓度、反应时间等条件进行优化,并对该方法的灵敏度、准确性、特异性进行评估,将其应用于食品中苏丹红Ⅲ的快速检测,并与国标法对比验证。结果显示,在适配体浓度100 nmol/L、NaCl浓度40 mmol/L、反应时间10 min等最优条件下,纳米金吸光度比值(A_(620)/A_(520))与苏丹红浓度在1.8~57.6 ng/mL范围内呈良好线性关系(R^(2)=0.981),方法检出限范围0.76~1.12 ng/mL,裸眼可视化检出限为7.2 ng/mL,检测时间为12 min。特异性分析显示,本方法对苏丹红Ⅰ,Ⅱ和Ⅳ有交叉反应,表明可以用于检测苏丹红Ⅰ~Ⅳ。食品中苏丹红Ⅲ加标回收率为82.1%~94.7%,相对标准偏差3.9%~7.7%,与国标方法相比无显著差异(P>0.05)。该方法适用于批量样品中苏丹红的快速检测。A colorimetric assay was prepared for SudanⅢdetection using aptamer as recognition element,gold nanoparticles as color indicator,and NaCl solution as an inducer for gold nanoparticle aggregation.Several crucial parameters that influenced sensitivity were investigated,including NaCl concentration,aptamer concentration,and the reaction time.The developed assay was applied to detect SudanⅢin food samples,and was validated using GB standard method.The results indicated that the linear relationship between concentration of Sudan dyes and absorbance ratio of gold nanoparticles(A_(650)/A_(520))was in the range of 1.8 to 57.6 ng/mL under the optimal experimental conditions:the concentrations of aptamer and salt of 100 nmol/L and 40 mmol/L,and the reaction time of 10 min.The detection limits ranged from 0.76 to 1.12 ng/mL by the instrument,and the limit of visual detection was 7.2 ng/mL by naked-eye observation.In addition,the detection time was 12 min.Specificity analysis showed that this method had obvious cross reactivity for SudanⅠ,ⅡandⅣ,indicating that the developed method can be used to detect SudanⅠ-Ⅳ.Furthermore,the developed assay was applied to spiked food samples and the recoveries of SudanⅢwere in the range of 82.1%-94.7%,with the relative standard deviations of 3.9%-7.7%.The test results obtained by the proposed method were in consistency with those obtained from GB standard method,with no significant differences(P>0.05).The developed method could be a potential screening tool for the detection of Sudan in food samples.

关 键 词：苏丹红 适配体 纳米金 比色分析 

分 类 号：O657.3[理学—分析化学]