基于微流控芯片的肿瘤细胞体外药敏试验研究进展  

Research progress on in-vitro drug sensitivity testing for tumor cells based on microfluidic chip

作　　者：赵明威 郭世伟 戴博[1] 李振庆[1] 郑璐璐 张大伟[1] 陶春先[1] ZHAO Mingwei;GUO Shiwei;DAI Bo;LI Zhenqing;ZHENG Lulu;ZHANG Dawei;TAO Chunxian(Engineering Research Center of Optical Instruments and Systems(MOE),School of Optical-Electrical and Computer Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;Department of Pancreatic Hepatobiliary Surgery,Changhai Hospital Affiliated to Naval Military Medical University,Shanghai 200433,China;Shanghai Environmental and Biosafety Instrument and Equipment Engineering Research Center,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学光电信息与计算机工程学院,光学仪器与系统教育部工程研究中心,上海200093 [2]海军军医大学附属长海医院,胰腺肝胆外科,上海200433 [3]上海理工大学,上海市环境与生物安全仪器设备工程研究中心,上海200093

出　　处：《分析试验室》2023年第2期266-274,共9页Chinese Journal of Analysis Laboratory

基　　金：上海市自然科学基金(19ZR1477500);上海市军民融合发展专项资金科技创新支持项目(2020-jmrh-kj8)资助。

摘　　要：在抗癌药物研发及疾病治疗过程中,药物溶液实现梯度浓度及混合配置,对确定最佳浓度和联合治疗方案至关重要。微流控技术通过设定微通道网络实现复杂的溶液处理,可进行药理学分析。微流控技术是构建体外微环境、开展细胞培养和药敏研究的重要手段。本文针对肿瘤细胞体外药敏试验研究,在微流控技术原理、制造材料、结构设计和制造特点等方面,对近10年来微流控芯片功能结构、在细胞体外培养和药物筛选应用中的研究进展做了概括和综述,对未来用于癌症个性化治疗的微流控芯片结构和功能进行了展望。In the development of anti-cancer drugs and treatment of diseases,realizing gradient concentrations and mixing configurations of drug solutions is important for determining the optimal concentration and combination therapy plan.Microfluidic technology is capable of processing complex solutions by setting up a network of microchannels,which can perform pharmacological analyses.Microfluidic technology plays an important role in the generation of in-vitro microenvironment,conduction of cell culture and drug susceptibility test.Herein,the research on in-vitro drug sensitivity testing for tumor cells is summarized.We reviewed the development in the construction of functional structures,applications of cell culture in vitro and drug screening in the aspect of the principle of microfluidic technology,manufacturing materials,structure design and fabrication in rencent ten years.The structures and functions of microfluidic chips for personalized cancer treatment in the future are prospected.

关 键 词：癌细胞 体外培养 药敏试验 微流控芯片 通道网络 

分 类 号：R965.1[医药卫生—药理学]