蚯蚓生物滤池削减剩余污泥中ARGs和ARB的效果研究  

REMOVAL EFFECT OF VERMIFILTRATION ON ARGs AND ARB IN EXCESS SLUDGE

作　　者：赵然 邢美燕[1] 杨格格 邢丽波 ZHAO Ran;XING Meiyan;YANG Gege;XING Libo(College of Environmental Science and Engineering,Tongji University,Shanghai 200092,China;Tongji Architectural Design(Group)Co.,Ltd,Shanghai 200092,China)

机构地区：[1]同济大学环境科学与工程学院,上海200092 [2]同济大学建筑设计研究院(集团)有限公司,上海200092

出　　处：《环境工程》2022年第12期112-120,共9页Environmental Engineering

基　　金：同济大学“双带头人”学术能力提升计划(0400219423);国家自然科学基金(51679168)。

摘　　要：蚯蚓生物滤池(vermifiltration, VF)是一种低碳、环境友好的生态技术。基于其良好的剩余污泥减量化效果,以不加蚯蚓的普通生物滤池(biofilter, BF)为对照,探究蚯蚓生物滤池对新型污染物——抗生素抗性菌(antibiotic resistance bacteria, ARB)和抗生素抗性基因(antibiotic resistance genes, ARGs)的去除效果。结果表明:与BF相比,VF对ARB(四环素类抗性菌、磺胺类抗性菌和双抗性抗性菌)的去除率增加25百分点以上,对ARGs(tetO、tetM、tetQ、tetW、sul1、sul2)去除率增加20百分点以上,且具有选择性。VF对intI1去除率高达98.24%,大大降低了ARGs的水平转移和传播风险。夏、冬季,VF对ARGs和ARB去除率比BF高15百分点以上,说明蚯蚓的加入减弱了低、高温对生物滤池的抑制作用。蚯蚓生物滤池能有效提高污泥中ARGs和ARB去除率,为剩余污泥的无害化处理提供参考。Vermifiltration(VF) is a low-carbon and eco-friendly technology. Based on the excellent excess sludge reduction efficacy, the removal effects of newly emerging pollutants, antibiotic resistance genes(ARGs) and antibiotic resistant bacteria(ARB) were evaluated, with a conventional biofilter(BF, no earthworm) as a control. The results indicated that the VF increased the removal rate of ARB(tetracycline resistant bacteria, sulfamethoxazole resistance bacteria and double resistance bacteria) by more than 25%, and exhibited higher but selective ARGs(tetO, tetM, tetQ, tetW, sul1, sul2) removal rates, generally increased by more than 20%, compared to the BF. Moreover, the average removal rate of intI1 by VF was 98.24%, reducing the risk of horizontal transmission of ARGs. In summer and winter, compared with the BF, the removal of ARGs and ARB by the VF increased by more than 15%, indicating that the addition of earthworms weakened the inhibitory effect of low and high temperatures. Conclusively, vermifiltration could effectively improve the removal rates of various ARGs and ARB in the excess sludge by the excellent excess sludge removal, which provides a reference for the ARGs and ARB removal assisted by the earthworms.

关 键 词：蚯蚓生物滤池 ARGs ARB 定量PCR 

分 类 号：X703[环境科学与工程—环境工程]