Highly efficient generation of blastocyst-like structures from spliceosomes-repressed mouse totipotent blastomere-like cells  

作　　者：Pengfei Zhang Xuzhao Zhai Boyan Huang Shu Sun Wen Jing Wang Man Zhang 

机构地区：[1]GMU-GIBH Joint School of Life Sciences,The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and Diseases,The Fifth Affiliated Hospital of Guangzhou Medical University,Guangzhou Laboratory,Guangzhou Medical University,Guangzhou 510005,China [2]Center for Cell Lineage and Atlas(CCLA),Bioland Laboratory,Guangzhou Regenerative Medicine and Health Guangdong Laboratory,Guangzhou 510005,China

出　　处：《Science China(Life Sciences)》2023年第3期423-435,共13页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(32070800)。

摘　　要：Mammalian embryogenesis begins with a totipotent zygote.Blastocyst-like structures can be captured by aggregated cells with extended pluripotent properties in a three-dimensional(3D)culture system.However,the efficiency of generating blastoids is low,and it remains unclear whether other reported totipotent-like stem cells retain a similar capacity.In this study,we demonstrated that spliceosomal repression-induced totipotent blastomere-like cells(TBLCs)form blastocyst-like structures within around 80%of all microwells.In addition,we generated blastoids initiating from a single TBLC.TBLC-blastoids express specific markers of constituent cell lineages of a blastocyst and resemble blastocyst in cell-lineage allocation.Moreover,singlecell RNA sequencing revealed that TBLC-blastoids share a similar transcriptional profile to natural embryos,albeit composed of fewer primitive endoderm-like cells.Furthermore,TBLC-blastoids can develop beyond the implantation stage in vitro and induce decidualization in vivo.In summary,our findings provided an alternative cell type to efficiently generate blastoids for the study of early mouse embryogenesis.

关 键 词：blastoids TBLCs early embryogenesis totipotent 

分 类 号：Q81[生物学—生物工程]