A novel hybrid seed production technology based on a unilateral cross-incompatibility gene in maize  

作　　者：Darun Cai Zhaogui Zhang Li Zhao Juan Liu Huabang Chen 

机构地区：[1]State Key Laboratory of Plant Cell and Chromosome Engineering,Innovative Academy of Seed Design,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China [2]University of Chinese Academy of Sciences,Beijing 100039,China

出　　处：《Science China(Life Sciences)》2023年第3期595-601,共7页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(32101725,32172058)。

摘　　要：Hybrid seed production technology(SPT)using genic recessive male sterility is of great importance in maize breeding.Here,we report a novel SPT based on a maize unilateral cross-incompatibility gene Zm Ga1F with an extremely low transgene transmission rate(TTR).Proper pollen-specific Zm Ga1F expression severely inhibits pollen tube growth leading to no fertilization.The maintainer line harbors a transgene cassette in an ipe1 male sterile background containing IPE1 to restore ipe1 male fertility,Zm Ga1F to prevent transgenic pollen escape,the red fluorescence protein encoding gene Ds Red2 for the separation of male sterile and fertile seeds,and the herbicide-resistant gene Bar for transgenic plant selection.When the maintainer line is selfed,gametes of ipe1/transgene and ipe1/-genotypes are produced,and pollen of the ipe1/transgene genotype is not able to fertilize female gametes due to pollen tube growth inhibition by Zm Ga1F.Subsequently,seeds of ipe1/ipe1 and ipe1/transgene genotypes are produced at a 1:1 ratio and could be separated easily by fluorescence-based seed sorting.Not a single seed emitting fluorescence is detected in more than 200,000 seeds examined demonstrating that the pollen-tube-inhibition(PTI)-based TTR is lower than what has been reported for similar technologies to date.This PTI-based SPT shows promising potential for future maize hybrid seed production.

关 键 词：ZmGa1F pollen tube growth genic male sterility seed production technology transgene transmission rate 

分 类 号：S513[农业科学—作物学]