Highly efficient base editing in rabbit by using near-PAMless engineered CRISPR/Cas9 variants  

作　　者：Zhongtian Zhang Xinyu Wu Jie Yang Xin Liu Ruonan Liu Yuning Song 

机构地区：[1]Jilin Provincial Key Laboratory of Animal Embryo Engineering,College of Veterinary Medicine,Jilin University,Changchun 130062,China

出　　处：《Science China(Life Sciences)》2023年第3期635-638,共4页中国科学（生命科学英文版）

基　　金：supported by the National Key Research and Development Program of China Stem Cell and Translational Research(2019YFA0110700);the National Natural Science Foundation of China(32000359)。

摘　　要：Dear Editor,Base editors can achieve targeted C-to-T and A-to-G conversion without the generation of DNA double-strand breaks(DSBs)or the requirement of a donor template,showing the potential to generate new mutations or to correct pathogenic mutations(Gaudelli et al.,2018).However,for conventional base editors,efficient base editing requires the presence of an NGG protospacer adjacent motif(PAM)that lies 12–16 nt downstream of the target bases,which generally limits the application of these base editors(Gaudelli et al.,2018).

关 键 词：BASE RABBIT BASES 

分 类 号：Q78[生物学—分子生物学]