Preparation and cellular uptake behaviors of uniform fiber-like micelles with length controllability and high colloidal stability in aqueous media  

作　　者：Junyu Ma Chen Ma Xiaoyu Huang Pedro Henrique Hermes de Araujo Amit Kumal Goyal Guolin Lu Chun Feng 

机构地区：[1]Key Laboratory of Synthetic and Self-Assembly Chemistry for Organic Functional Molecules,Center for Excellence in Molecular Synthesis,Shanghai Institute of Organic Chemistry,University of Chinese Academy of Sciences,Chinese Academy of Sciences,345 Lingling Road,Shanghai 200032,China [2]Department of Chemical Engineering and Food Engineering,Federal University of Santa Catarina,Florianópolis-SC,88040-970,SC,Brazil [3]Department of Pharmacy,School of Chemical Sciences and Pharmacy,Central University of Rajasthan,Tehsil-Kishangarh-305801 Distt.-Ajmer,Rajasthan,India

出　　处：《Fundamental Research》2023年第1期93-101,共9页自然科学基础研究（英文版）

基　　金：The authors are thankful for financial support from National Science Foundation for Distinguished Young Scholars(51825304);National Natural Science Foundation of China(52122314,51873229 and 51961145103);the project of Bureau of International Cooperation,CAS(121731KYSB20200006);Youth Innovation Promotion Association of CAS(Y2020062);Shanghai Scientific and Technological Innovation Project(19ZR1468400,19590750400,20JC1415400 and 21520780100);Innovation Program of Shanghai Municipal Education Commission(2019-01-07-00-05-E00012).

摘　　要：Fragmentation/disassembly of fiber-like micelles generated by living crystalline-driven self-assembly(CDSA)is usually encountered in aqueous media,which hinders the applications of micelles.Herein,we report the generation of uniform fiber-like micelles consisting of a𝜋Л-conjugated oligo(p-phenylenevinylene)core and a crosslinking silica shell with grafted poly(ethylene glycol)(PEG)chains by the combination of living CDSA,silica chemistry and surface grafting-onto strategy.Owing to the presence of crosslinking silica shell and the outmost PEG chains,the resulting micelles exhibit excellent dispersity and colloidal stability in PBS buffer,BSA aqueous solution and upon heating at 80℃ for 2 h without micellar fragmentation/disassembly.The micelles also show negligible cytotoxicity toward both HeLa cervical cancer and HEK239T human embryonic kidney cell lines.Interestingly,micelles with Ln of 156 nm show the“stealth”property with no significant uptake by HeLa cells,whereas some certain amounts of micelles with Ln of 535 nm can penetrate into HeLa cells,showing length-dependent cellular uptake behaviors.These results provide a route to prepare uniform,colloidally stable fiber-like nanostructures with tunable length and functions derived for biomedical applications.

关 键 词：Fiber-like nanostructures Colloidal stability Living crystallization-driven self-assembly Silica shell Cellular uptake 

分 类 号：TB3[一般工业技术—材料科学与工程]