抗感玉米自交系对小斑病菌早期侵染反应的转录组分析  

Transcriptome Analysis of Resistant and Susceptible Inbred Line of Maize(Zea mays)in Response to Early Infection of Cochliobolus heterostrophus

作　　者：甘林[1] 兰成忠[1] 阮妙鸿 刘晓菲 黄伟群[2] 代玉立[1] 杨秀娟[1] GAN Lin;LAN Cheng-Zhong;RUAN Miao-Hong;LIU Xiao-Fei;HUANG Wei-Qun;DAI Yu-Li;YANG Xiu-Juan(Institute of Plant Protection/Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China;Fujian Seed Station,Fuzhou 350001,China)

机构地区：[1]福建省农业科学院植物保护研究所/福建省作物有害生物监测与治理重点实验室,福州350013 [2]福建省种子总站,福州350001

出　　处：《农业生物技术学报》2023年第3期460-474,共15页Journal of Agricultural Biotechnology

基　　金：国家重点研发计划(2018YFD0200706);福建省属公益类科研院所专项(2018R1025-1);福建省农业科学院植物保护创新团队(CXTD2021027);福建省农业科学院“5511”协同创新工程(XTCXGC2021011);福建省农业科学院科技创新平台专项(CXPT202105)。

摘　　要：玉米小斑病是世界玉米(Zea mays)产区重要的一种叶部真菌性病害,不同遗传背景的玉米品种对小斑病的抗性存在明显差异。为探明玉米对小斑病菌(Cochliobolus heterostrophus)早期侵染反应的分子机制,本研究采用比较转录组测序技术对小斑病菌接种12和24 h后的抗病自交系'Mo17-R'和感病自交系'罗31-S'的测序数据进行了分析。结果表明,接种病原菌后,'Mo17-R'和'罗31-S'分别共有11416和11027个差异表达基因。通过GO功能富集和KEGG代谢途径分析,发现两个玉米自交系在GO分类的细胞、细胞部分、细胞器、膜、结合、催化活性、代谢过程、细胞过程和单有机物过程中被富集的差异表达基因较多;而在苯丙烷类生物合成、信号传导、植物-病原菌互作、淀粉和蔗糖代谢途径中也富集较多的差异表达基因。其中,与抗病相关的富集途径中,苯丙烷类生物合成、植物激素信号传导、植物-病原菌互作为'Mo17-R'和'罗31-S'两个自交系中差异基因共同显著富集的途径,而参与萜类合成途径的基因仅在感病自交系'罗31-S'中获得显著富集。此外,发现过氧化物酶、肉桂醇脱氢酶、乙烯应答转录因子、环核苷酸门控离子通道、细胞色素P450等相关基因在抗病自交系'Mo17-R'中被显著诱导上调表达,而在感病自交系'罗31-S'中基因诱导表达倍数低于抗病自交系或下调表达,提示这些基因在抗病玉米抵抗小斑病菌的早期侵染中发挥重要作用。本研究结果为深入探讨玉米和小斑病菌互作及玉米抗病分子机制提供重要的理论基础。Southern leaf blight was an important disease in maize planting region of the world.There were significant differences in resistance of maize(Zea mays)cultivar with different genetic backgrounds to the disease.In order to understand response profiles for gene expression in maize to early infection of Cochliobolus heterostrophus,transcriptional data in the resistant cultivar('Mo17-R')and susceptible cultivar('Luo31-S')of maize inbred line at 12 and 24 h after inoculation were analyzed using the transcriptome analyses.The results demonstrated that 11416 and 11027 differentially expressed genes(DEGs)were found in'Mo17-R'and'Luo31-S'after inoculation by C.heterostrophus,respectively.GO enrichment and KEGG metabolic pathway analysis revealed that the DEGs were most rich in the terms of cell,cell part,organelle,membrane,binding,catalytic activity,metabolic process,cellular process and single-organism process,which were also most rich in the pathways of phenylpropanoid biosynthesis,signal transduction,plant-pathogen interaction,starch and sucrose metabolism.Among the enriched pathways associated with resistance,the pathways of phenylpropanoid biosynthesis,plant hormone signal transduction and plant-pathogen interaction were common in'Mo17-R'and'Luo31-S',while the DEGs involved in terpenoid biosynthesis pathway were only enriched in'Luo31-S'.Furthermore,theses DEGs related to peroxidase,cinnamyl alcohol dehydrogenase,ethylene-responsive transcription factor,cyclic nucleotide-gated ion channel,cytochrome P450 were specifically induced in the resistant inbred line('Mo17-R'),while these DEGs were down-regulated or less induced in the susceptible inbred line('Luo31-S').It was speculated that these genes could play an important role in disease resistance for resistant maize at the early stage of C.heterostrophus infection.These results provides a theoretical basis for further exploitation of molecular mechanisms underlying the interaction between maize and C.heterostrophus,and maize resistance to the pathogen.

关 键 词：玉米 小斑病菌 转录组 基因表达 互作机制 抗病性 

分 类 号：S432.4[农业科学—植物病理学]