油松镰刀菌四个Rab蛋白的生物信息学分析  

Bioinformatics Analysis on Four Rab Proteins of Fusarium Infecting Pinus Tabulaeformis

作　　者：张春源 赖泽成 吕正阳 邵登科 刘梓烨 吕祎凡 易听雨 许钰滢 叶文雨[1,2] ZHANG Chun-yuan;LAI Ze-cheng;LV Zheng-yang;SHAO Deng-ke;LIU Zi-ye;LV Yi-fan;YI Ting-yu;XU Yu-ying;YE Wen-yu(Fujian Agriculture&Forestry University,China National Engineering Research Center of JUNCAO Technology,Fuzhou Fujian 350002,China;Fujian University Key Laboratory for Plant-Microbe Interaction,College of Life Science,Fujian Agriculture&Forestry University,Fuzhou Fujian 350002,China;College of Future Technology(Haixia Institute of Science and Technology),Fujian Agriculture&Forestry University,Fuzhou Fujian 350002,China)

机构地区：[1]福建农林大学国家菌草工程技术研究中心,福建福州350002 [2]植物与微生物相互作用福建省高校重点实验室,福建农林大学生命科学学院,福建福州350002 [3]福建农林大学未来技术学院(海峡联合研究院),福建福州350002

出　　处：《青海农林科技》2023年第1期80-86,共7页Science and Technology of Qinghai Agriculture and Forestry

基　　金：福建农林大学科技创新专项基金项目(No.KFA19137A);福建农林大学科技创新专项基金项目(No.KFA19136A);福建省自然资源科技创新项目资金资助(KY-090000-04-2022-016);福建省科技重大专项(2021NZ029009);福建省引导性科技计划项目(2022N0010)。

摘　　要：本文利用生物信息学方法,分析了一种可侵染油松针叶的病原真菌镰刀菌四个Rab蛋白Fp_xl1、Fp_xl2、Fp_xl3、Fp_xl4的理化性质、跨膜结构、信号肽、蛋白结构和蛋白互作关系。结果表明:Fp_xl1、Fp_xl2、Fp_xl3、Fp_xl4均为无跨膜结构、不含信号肽、不稳定的亲水蛋白,除Fp_xl2是酸性蛋白质外其他三个均属于碱性蛋白质;四个蛋白中均存在较多的α螺旋与无规则卷曲结构域,其中Fp_xl2稳定性最高,Fp_xl3稳定性最低;Fp_xl1和Fp_xl3可与含蛋白激酶结构域的蛋白质互作,Fp_xl2和Fp_xl3可与含Rab-GAP TBC结构域的蛋白互作,而Fp_xl4能与含SEC7结构域的蛋白质互作。In this study,the physical and chemical properties,transmembrane domains,signal peptides,structure and protein-protein interaction of four Rab proteins from Fusarium infecting Pinus tabulaeformis were analyzed by the bioinformatic approaches.Four proteins were named as Fp_xl1,Fp_xl2,Fp_xl3,Fp_xl4,respectively.The results showed that Fp_xl1,Fp_xl2,Fp_xl3 and Fp_xl4 were basically unstable hydrophilic proteins and none of them had signal peptides or transmembrane domains.Fp_xl2 was an acidic protein,while the other three proteins were basic proteins.There were manyαhelix and random coil domains in all the four Rab proteins.Among them,Fp_xl2 had the highest stability while Fp_xl3 had the lowest.Protein-protein interaction analysis showed that Fp_xl1 and Fp_xl2 could interact with protein kinase domain-containing proteins;Fp_xl2 and Fp_xl3 could interact with Rab-GAP TBC domain-containing proteins;Fp_xl4 could interact with SEC7 domain-containing proteins.

关 键 词：园林植物 镰刀菌 RAB蛋白 生物信息学 

分 类 号：Q811.4[生物学—生物工程]