比较两种γ-干扰素释放试验对结核分枝杆菌感染的诊断价值  

A Comparison Study of the Diagnostic Value of Two Gamma-Interferon Release Assays for Mycobacterium Tuberculosis Infection

作　　者：赵志鹏[1] 李晓晨 熊攀 林明贵 赵秀英[1] ZHAO Zhipeng;LI Xiaochen;XIONG Pan;LIN Minggui;ZHAO Xiuying(Beijing Tsinghua Changgung Hospital,School of Clinical Medicine,Tsinghu University,Beijing 100044,China)

机构地区：[1]清华大学附属北京清华长庚医院,清华大学临床医学院检验医学科,北京102218

出　　处：《标记免疫分析与临床》2022年第12期2116-2120,共5页Labeled Immunoassays and Clinical Medicine

基　　金：北京清华长庚医院研究基金资助(编号:12015C1040)。

摘　　要：目的分析两种结核特异性γ-干扰素释放试验在辅助诊断结核感染中的应用价值。方法对平行对照组(同时进行了A、B两种方法检测)、临床病例组(部分进行了A方法检测,另外一部分进行了B方法检测),两组检测结果与临床诊断结果进行比对,评估两种方法在结核感染性疾病的辅助诊断中价值差异。进一步将临床病例组分为肺结核组和肺外结核组,比较了两种方法在肺内和肺外结核感染诊断中的应用价值。结果A方法与临床诊断的一致性、灵敏度、特异性均高于B方法;两种方法对肺结核诊断的灵敏度均优于对肺外结核的诊断,但A方法阳性率高于B方法。结论在辅助临床诊断结核感染中,无论肺结核感染还是肺外结核感染,A方法的价值均优于B方法。A方法值得试验室优先选择。Objective To compare the application values of twoγ-interferon release assays in assisting the diagnosis of tuberculosis infection.Methods Two groups of data were collected for the study,including:parallel control group(both A and B methods were tested simultaneously)and clinical case group(some of which were tested by method A and the other part were tested by method B).The results were compared with the clinical diagnosis to evaluate the diagnostic value of the two methods in tuberculosis infectious diseases.The clinical case group was further divided into the intrapulmonary tuberculosis group and extrapulmonary tuberculosis group,and the application value of the two methods in the diagnosis of intrapulmonary tuberculosis infection and extrapulmonary tuberculosis infection was compared.Results The consistency with clinical diagnosis,sensitivity and specificity of method A were higher than that of method B.Both methods were more sensitive to the diagnosis of intrapulmonary tuberculosis than extrapulmonary tuberculosisbut.Method A had a higher sensitivity than method B.Conclusion In the auxiliary clinical diagnosis of tuberculosis infection,for both intrapulmonary tuberculosis infection and extrapulmonary tuberculosis infection,the value of method A is superior to method B,suggesting that and method A deserves the priority choice for laboratory application.

关 键 词：结核感染 Γ-干扰素释放试验 干扰素-Γ 融合蛋白 ESAT-6-CFP-10-Rv1985c 

分 类 号：R378.911[医药卫生—病原生物学]