中国北方玉蜀黍黑粉菌交配型a位点鉴别及遗传多样性分析  

Mating Type a Locus Identification and Genetic Diversity Analysis of Ustilago maydis in North China

作　　者：刘小迪 马周杰 何世道 黄宇飞[1] 姚远[1] 孙艳秋[1] 高增贵[1] LIU Xiao-di;MA Zhou-jie;HE Shi-dao;HUANG Yu-fei;YAO Yuan;SUN Yan-qiu;GAO Zeng-gui(College of Plant Protection,Shenyang Agricultural University,Shenyang 110161,China)

机构地区：[1]沈阳农业大学植物保护学院,沈阳110161

出　　处：《玉米科学》2023年第1期153-160,共8页Journal of Maize Sciences

基　　金：国家重点研发计划课题项目(2018YFD0300307,2017YFD0300704,2016YFD0300704)。

摘　　要：利用特异性引物对2017年采自7个省(自治区)的84份玉米瘤黑粉病菌进行交配型a位点鉴别,采用相关序列扩增多态性(ISSR)分子标记技术对供试菌株进行遗传多样性分析,分析我国北方玉米主要产区玉蜀黍黑粉菌交配型a位点基因型种类及其遗传多样性水平。结果表明,玉蜀黍黑粉菌交配型a位点基因型主要有mfa1、mfa2和mfa1+mfa2三个种类,84株菌株中,mfa1基因型和mfa1+mfa2基因型均有31株,占鉴定株数的36.9%;22株为mfa2位点,占鉴定株数的26.2%。通过筛选出的8条ISSR引物共扩增出61条清晰条带,特异性条带55条,多态性位点所占比例为90.2%。UPGMA聚类分析结果表明,供试菌株遗传相似系数介于0.59~0.92,在遗传相似系数为0.65时供试菌株被分为6个类群,表现出丰富的遗传多样性。ISSR揭示的玉蜀黍黑粉菌遗传多样性、交配型a位点基因型以及地理来源之间的关系分析结果表明,菌株的遗传类群和交配型a位点基因型之间无密切关系,并且两者与菌株地理来源也无明显相关性。To clarify the mating type a locus and the genetic diversity of Ustilago maydis in the main corn pro⁃ducing areas in North China.The mating type a locus identification and genetic diversity analysis of 84 corn smut isolates collected from 7 provinces(autonomous regions)were carried out by the inter simple sequence repeat(ISSR)molecular marker technology.The results showed that there were three genotypes of mating type a locus of Ustilago maydis,mfa1,mfa2 and mfa1+mfa2.In the 84 isolates,mfa1 genotype and mfa1+mfa2 genotype both had 31 iso⁃lates,accounting for 36.9%of the total;22 isolates were mfa2,accounting for 26.2%.The results demonstrated that 55 specific bands were amplified by 8 primers in the 61 bands,the proportion of loci was 90.2%.UPGMA cluster analysis showed that the genetic similarity coefficient of the tested isolates ranged from 0.59 to 0.92,and when the threshold was 0.65,all isolates were divided into 6 groups,showing rich genetic diversity.The analysis results of the relationship among the genetic diversity,genotypes of mating type a locus and geographical origins of Ustilago may⁃dis revealed by ISSR indicated that there was not a close relationship between the genetic groups and genotypes of mating type a locus of the tested isolates,and there was no obvious correlation between them and the geographical origins.

关 键 词：玉米 玉蜀黍黑粉菌 交配型a位点 ISSR 遗传多样性 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]