甘肃省辣椒斑萎病毒的检测  

Detection of Pepper Spotted Wilt Virus in Gansu Province

作　　者：陈灵芝[1] 王兰兰[1] 张茹[1] 张玉鑫[1] 高彦萍 CHEN Lingzhi;WANG Lanlan;ZHANG Ru;ZHANG Yuxin;GAO Yanping(Institute of Vegetables,Gansu Academy of Agricultural Sciences,Lanzhou 730070,Gansu,China;Gansu Potato Seed(Seedling)Virus Detection and Safety Evaluation of Engineering Technology Research Center,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃省农业科学院蔬菜研究所,甘肃兰州730070 [2]甘肃省马铃薯脱毒种薯(种苗)病毒检测及安全评价工程技术研究中心,甘肃兰州730070

出　　处：《中国蔬菜》2023年第3期73-77,共5页China Vegetables

基　　金：甘肃省农业科学院生物育种项目(2020GAAS09);甘肃省重点研发计划项目(22YF7NA033)。

摘　　要：在甘肃省兰州、白银、庆阳采集发病症状表现为植株矮化、叶片黄化、生长点坏死的辣椒病样,采用RT-PCR法和简易试纸条进行病毒检测。结果表明:通过RT-PCR法共检测出了6种病毒:辣椒环斑病毒(ChiRSV)、辣椒轻斑驳病毒(PMMoV)、辣椒斑驳病毒(PepMoV)、辣椒叶脉黄化病毒(PeVYV)、烟草轻绿花叶病毒(TMGMV)、番茄斑萎病毒(TSWV);且在同一个病样上,存在多种病毒复合侵染的状况。利用简易试纸条进行检测,只检测出了TSWV,但检出率较高。表明,甘肃地区辣椒生产上侵染的病毒种类多,TSWV的发生越来越普遍和严重。Using RT-PCR methods and simple test strip detection,this study detected pepper pathogenic virus in disease infected samples with symptoms of plant dwarfing,leaf yellowing and growth point necrosis collected from Lanzhou,Baiyin and Qingyang areas of Gansu Province.The results showed that 6 viruses were detected by RT-PCR:chilli ringspot virus(ChiRSV),pepper mild mottle virus(PMMoV),pepper mottle virus(PepMoV),pepper vein yellows virus(PeVYV),tobacco mild green mosaic virus(TMGMV),tomato spotted wilt virus(TSWV).There were multiple virus infection status on the same sample.Simple test strips were used for further detection,only TSWV were detected.But the detection rate was high,indicating that there were many kinds viruses infected in pepper production in Gansu region,and TSWV occurrence was becoming more and more common and serious.

关 键 词：辣椒 番茄斑萎病毒 RT-PCR检测 简易试纸条检测 

分 类 号：S436.418.12[农业科学—农业昆虫与害虫防治]