不同处理措施对辣椒连作土壤细菌群落结构及多样性的影响  

Effects of Different Treatments on Structure and Diversity of Soil Bacterial Community in Pepper Continuous Cropping Soil

作　　者：薛国萍 白红梅 杜金伟 朱春侠 付崇毅 姜伟 宋庆成 李杰 郭欢欢 张晓梅 XUE Guoping;BAI Hongmei;DU Jinwei;ZHU Chunxia;FU Chongyi;JIANG Wei;SONG Qingcheng;LI Jie;GUO Huanhuan;ZHANG Xiaomei(Inner Mongolia Academy of Agricultural&Animal Husbandry Sciences,Hohhot 010031,Inner Mongolia,China;Ningcheng County Agriculture and Animal Husbandry Technology Promotion Center,Ningcheng 024200,Inner Mongolia,China;Chifeng Academy of Agricultural and Animal Husbandry Sciences,Chifeng 024031,Inner Mongolia,China;Tongliao Agricultural Technology Promotion Center,Tongliao 028000,Inner Mongolia,China)

机构地区：[1]内蒙古自治区农牧业科学院,内蒙古呼和浩特010031 [2]宁城县农牧技术推广中心,内蒙古宁城024200 [3]赤峰市农牧科学研究所,内蒙古赤峰024031 [4]通辽市农业技术推广中心,内蒙古通辽028000

出　　处：《中国蔬菜》2023年第3期78-84,共7页China Vegetables

基　　金：内蒙古自治区科技计划项目(2022YFDZ0052);中央农业重大技术协同推广计划项目;第十一批草原英才创新人才团队项目。

摘　　要：以辣椒连作20年的土壤为研究对象,设置腐熟玉米秸秆还田、小麦填闲、添加抗重茬生物菌肥、威百亩熏蒸消毒等4个处理,以不做任何处理的辣椒连作土壤为对照,探究不同处理措施对辣椒连作土壤细菌群落结构组成和多样性的影响。结果表明,腐熟玉米秸秆还田、小麦填闲、添加抗重茬生物菌肥处理均显著增加了土壤细菌群落Chao1指数、Shannon指数,以添加抗重茬生物菌肥处理的多样性指数最高。不同处理对辣椒连作土壤细菌群落结构均具有极显著影响。门水平上,腐熟玉米秸秆还田、小麦填闲处理显著增加了芽单胞菌门(Gemmatimonadetes)相对丰度;腐熟玉米秸秆还田、威百亩熏蒸消毒处理显著增加了放线菌门(Actinobacteria)相对丰度;4个处理均显著增加了疣微菌门(Verrucomicrobia)相对丰度;威百亩熏蒸消毒处理显著增加了厚壁菌门(Firmicutes)相对丰度,显著降低了变形菌门(Proteobacteria)相对丰度。属水平上,4个处理均显著增加了Flavisolibacter相对丰度;腐熟玉米秸秆还田、小麦填闲、添加抗重茬生物菌肥处理显著增加了类固醇杆菌属(Steroidobacter)、Terrimonas相对丰度;腐熟玉米秸秆还田、小麦填闲处理亦显著增加了MND1、赭黄嗜盐囊菌属(Haliangium)相对丰度;腐熟玉米秸秆还田、添加抗重茬生物菌肥处理显著增加了Subgroup_10、农研丝杆菌属(Niastella)相对丰度;小麦填闲处理显著增加了芽孢杆菌属(Bacillus)相对丰度;添加抗重茬生物菌肥显著增加了鞘氨醇单胞菌属(Sphingomonas)、假单胞菌属(Pseudomonas)和链霉菌属(Streptomyces)相对丰度。综上所述,腐熟玉米秸秆还田、小麦填闲、添加抗重茬生物菌肥处理效果较佳,威百亩熏蒸消毒处理效果最差。Taking soil with 20 years pepper continuous cropping as research object,this paper set up 4 treatments,including:returning decompoed corn straw to fields,wheat filling,adding anti-crop biological bacterial fertilizer,weibaimu fumigation and disinfection,etc.;and took pepper continuous cropping soil without any amendment as the contrast.The paper explored the effect of different treatments on composition of soil bacterial community and diversity in pepper continuous cropping soil.The results showed that returning decompoed corn straw to fields,wheat filling,adding anti-crop biological becterial bacterial fertilizer,all had significantly increased Chao1 index and Shannon index.And the diversity index of treatment by adding anti-crop biological bacterial fertilizer was the highest.Different treatments had extremely significant effect on bacterial community structure of pepper continuous cropping soil.At the phylum level,treatments of returning decompoed corn straw to fields and wheat filling had significantly increased the relative abundance Gemmatimonadetes.Treatment of returning decompoed corn straw to fields and weibaimu fumigation and disinfection had significantly increased the relative abundance of Actinobacteria.All these 4 treatments had significantly increased the relative abundance of Verrucomicrobia.Weibaimu fumigation and disinfection treatment had significantly increased the relative abundance of Firmicutes and decreased that of Proteobacteria.At the genus level,4 treatments had significantly increased the relative abundance of Flavisolibacter.Treatments of returning decompoed corn straw to fields,wheat filling and adding anticrop biological bacterial fertilizer had significantly increased the relative abundance of Steroidobacter and Terrimonas.Treatments of returning decompoed corn straw to fields,and wheat filling significantly increased the relative abundance of MND1 and Haliangium.Treatments of returning decompoed corn straw to fields,and adding anti-crop biological bacterial fertilizer had signific

关 键 词：辣椒 秸秆还田 小麦填闲 生物菌肥 威百亩熏蒸消毒 土壤细菌群落结构 

分 类 号：S641.3[农业科学—蔬菜学] S154.3[农业科学—园艺学]