BMPR-IB不同基因型对凉山黑绵羊不同生理时期主要生殖激素的影响  

Effects of BMPR-IB Different Genotypes on Main Reproductive Hormones in Liangshan Black Sheep at Different Physiological Stages

作　　者：王俊科 王娟红 常卫华 王晋康 滕悦扬 刘羽艳 王娇 曾玉婷 WANGJunke;WANG Juanhong;CHANGWeihua;WANG Jinkang;TENG Yueyang;LIU Yuyan;WANG Jiao;ZENG Yuting(College of Animal Science,Xichang University,Xichang615013,China;Panxi Laboratory of Animal Epidemic Disease Detection and Control of Sichuan,Xichang615013,China;Butuo County Agriculture and Rural Bureau,Butuo616350,China)

机构地区：[1]西昌学院动物科学学院,西昌615013 [2]四川攀西动物疫病检测与防控实验室,西昌615013 [3]布拖县农业农村局,布拖616350

出　　处：《中国草食动物科学》2023年第2期77-80,共4页China Herbivore Science

基　　金：西昌学院创新创业训练项目(西学院招[2022]6号);西昌学院博士基金项目(YBZ202132)。

摘　　要：为研究凉山黑绵羊在发情前期、发情期、发情后期和发情间期BMPR-IB基因不同基因型与生殖激素之间的调控关系,利用公羊试情法和外阴部观察法判断发情状态,采集凉山黑绵羊4个时期的颈静脉血,提取DNA,利用PCR扩增、酶切技术、琼脂糖凝胶电泳检测BMPR-IB基因的基因型,通过ELISA技术检测4种主要生殖激素在不同生理时期血液中的含量。结果显示,凉山黑绵羊BMPR-IB基因共有BB型、B+型和++型3种基因型。在发情期,FSH分泌量BB基因型显著高于B+基因型和++基因型(P<0.05);在发情前期,LH分泌量BB基因型显著高于B+基因型和++基因型(P<0.05);在发情中期和发情后期,P4分泌量BB基因型大于B+基因型和++基因型,且差异显著(P<0.05);E2分泌量在4个时期BMPR-IB基因不同基因型之间差异均不显著(P>0.05)。综上,凉山黑绵羊BMPR-IB存在多胎基因,而且有3种基因型;BMPR-IB基因的突变对FSH、LH和P4的分泌有促进作用。The aim of this study was to analyze the regulatory relationship between BMPR-IB different genotypes and reproductive hormones in Liangshan Black sheep during proestrus,estrus,metestrus and interestrus.Estrus state of Liangshan Black sheep was judged by the method of the ram testing and extrinsic observation.The neck venous blood was collected at four stages,and DNA was extracted.Then the BMPR-IB different genotypes were detected by PCR amplification,enzyme digestion technology and agarose gel electrophoresis.The blood levels of four major reproductive hormones in different physiological periods were detected by ELISA.The results showed that BB genotype,B+genotype and++genotype were detected in Liangshan Black sheep in this study.During the estrus period,the amount of FSH secreted by BB genotype was higher than that of B+genotype and++genotype(P<0.05).The amount of LH secreted of BB genotype was higher than that of B+genotype and++genotype(P<0.05).The secretion of P4 in BB genotype was higher than that in B+genotype and++genotype in mid and late estrus,and the difference was significant(P<0.05).But there was no significant difference in E2 content among the different genotypes of BMPR-IB gene in the four periods(P>0.05).In conclusion,there were multiple of BMPR-IB gene in Liangshan Black sheep,and had three genotypes.The mutation of BMPR-IB gene promoted the secretion of FSH,LH and P4.

关 键 词：凉山黑绵羊 BMPR-IB基因 激素 

分 类 号：S826.2[农业科学—畜牧学]