玉米雄性不育突变体x50的基因定位与遗传分析  

Gene Mapping and Genetic Analysis of Male Sterile Mutant x50 in Maize

作　　者：郭瑶晴 孙晓靖 连玉杰 陈慧 孙华越 张雪海 汤继华[1] 陈晓阳 GUO Yaoqing;SUN Xiaojing;LIAN Yujie;CHEN Hui;SUN Huayue;ZHANG Xuehai;TANG Jihua;CHEN Xiaoyang(College of Agronomy,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学农学院,河南郑州450046

出　　处：《华北农学报》2023年第1期17-22,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31801379);河南省自然科学基金项目(222300420186)。

摘　　要：为了挖掘雄性不育种质资源,鉴定雄性育性基因,为玉米雄性不育化制种提供基础材料。以玉米雄性不育突变体x50为试验材料,研究突变体雄性不育表型,构建x50与自交系Mo17的F1和F2群体,确定突变体x50雄性不育性状的遗传模式。以F2群体为材料,应用图位克隆技术定位雄性育性基因X50,通过基因等位性测验确定候选基因。结果显示,与野生型相比,雄性不育突变体x50花药不能从颖壳露出,花药体积较小且萎蔫,无成熟花粉粒形成。F1群体植株均表现为雄性可育,F2群体植株出现雄性育性分离,可育植株与不育植株分离比例符合3∶1,说明突变体x50不育性状受1对隐性核基因控制。通过图位克隆方法将雄性育性基因X50定位于玉米第2染色体分子标记2-4901与2-4963之间,物理区间为237.42~241.39 Mb。定位区间内候选基因分析发现,区间存在玉米雄性不育基因ZmMs33。以ms33纯合突变体ms33-6029和ms33-6052分别与x50杂合型+/x50杂交,杂交后代可育植株与不育植株分离比例符合1∶1,表明x50是ZmMs33基因一个等位突变体。玉米雄性不育突变体x50的鉴定为玉米杂交种子生产和ZmMs33基因功能研究提供了种质材料。In order to explore the germplasm resources of male sterile line and identify the male fertility genes,which would provide the basic materials for the maize seed production by the male sterile line.Using the male sterile mutant x50 as the experimental material,the male sterile phenotype of x50 was studied,and the F1and F2populations of x50 and inbred line Mo17 were constructed to determine the genetic pattern of the male sterile trait in x50.With the F2population,the male fertility gene X50 was identified by the map-based cloning.Furthermore,the candidate gene was confirmed by the allelism test.The results showed that compared with the wild type,the x50 anthers did not emerge from the glume,and was smaller and wilted,and no mature pollen grains were formed.All F1plants were fertile,and F2plants displayed 3∶1 segregation ratio between fertile and sterile plants,indicating that the monofactorial recessive inheritance of x50.The gene X50 was mapped to the interval from the molecular marker 2-4901 to 2-4963 on chromosome 2 with a physical range of 237.42 to 241.39 Mb by map-based cloning.Candidate gene analysis found that the male fertility gene ZmMs33 was located in the mapping region.In addition,the test crosses of the ms33 mutants(ms33-6029 and ms33-6052)and heterozygous+/x50 revealed 1∶1 segregation ratio between fertile and sterile plants.These results suggested that x50 was an allele mutant of ZmMs33 gene.The identification of the male sterile mutant x50 provided germplasm resources for maize hybrid seed production and functional study of ZmMs33 gene.

关 键 词：玉米 雄性不育基因X50 基因定位 花药 等位性测验 

分 类 号：S513[农业科学—作物学]