潞玉13亲本抗旱性比较分析  

Comparative Analysis of Drought Resistance of Luyu 13 Parents

作　　者：张文忠 赵晋锋 芦明 王慧慧 ZHANG Wenzhong;ZHAO Jinfeng;LU Ming;WANG Huihui(Institute of Millet,Shanxi Agricultural University,Changzhi 046011,China)

机构地区：[1]山西农业大学谷子研究所,山西长治046011

出　　处：《华北农学报》2023年第1期117-123,共7页Acta Agriculturae Boreali-Sinica

基　　金：山西农业大学生物育种工程项目(YZGC023);山西省自然科学研究项目(202103021224163);山西省现代农业技术体系项目(YMGW202206);山西省农业科学院项目(YGJPY2009)。

摘　　要：潞玉13是由山西农业大学谷子研究所育成的优良玉米品种。该品种抗旱性好、耐逆性强,社会效益显著。为比较杂交种潞玉13亲本1572和海921抗旱耐逆特性,采用20%PEG模拟干旱对潞玉13杂交种及双亲种子的萌发和幼苗生长相关参数进行了比较,结果表明,在正常条件下,潞玉13、1572和海921发芽参数无显著差别,但干旱条件下的发芽率、发芽势、发芽指数、萌发抗旱指数均受到抑制,而海921受抑制程度显著高于1572和潞玉13,揭示1572种子活力明显高于海921,在干旱胁迫下有较高的发芽率和发芽优势,对干旱有较强的适应能力。随后选取逆境应答关键基因ZmCIPK16和ZmSAM1,用荧光实时定量PCR方法研究它们在1572和海921自交系苗期不同非生物逆境胁迫以及不同生育期干旱胁迫下的表达情况,结果显示,ZmCIPK16和ZmSAM1广泛参与了1572和海921苗期干旱、盐、低温、高温和ABA以及在不同生育期的干旱应答,相比较在自交系1572中能更积极参与对干旱等逆境的响应。这些结果揭示自交系1572是优良的抗旱耐逆种质,可能在杂交种潞玉13抗旱耐逆方面贡献大于自交系海921。Luyu 13 is an excellent maize hybrid bred by the Millet Research Institute of Shanxi Agricultural University.It has strong drought and stress tolerance,and has achieved remarkable social benefits.In order to compare the drought and stress tolerance characteristics of the parents 1572 and Hai 921 of maize hybrid Luyu 13,we analyzed the related parameters of seed germination and seedling growth under simulated drought stress with 20%PEG solution.Results showed that there was no significant difference in the germination parameters between Luyu 13,1572 and Hai 921 under normal conditions,but the germination rate,germination energy,germination index and sprout index were inhibited under drought conditions,while the inhibition degree of Hai 921 was significantly higher than that of 1572 and Luyu 13.These results showed that the seed vigor of 1572 was higher than that of Hai 921,indicating inbred line 1572 had higher germination rate and germination advantage under drought stress,and possess stronger adaptability to drought.Subsequently,the important stresses response genes,ZmCIPK16 and ZmSAM1,were selected and analyzed their expression in 1572 and Hai 921 inbred lines under different abiotic stresses at seedling stage and under drought stress at different growth stages by Real-time PCR.Results showed that ZmCIPK16 and ZmSAM1 were widely responses to drought,salt,low temperature,high temperature,and ABA at seedling stage of 1572 and Hai 921 and responses to drought at different growth stages.ZmCIPK16 and ZmSAM1 showed more actively participated in the response to drought and other stresses in 1572.These results indicated that the inbred line 1572 was an excellent germplasm with drought and stress tolerance,and its contribution to drought resistance of hybrid Luyu 13 was greater than that of inbred line Hai 921.

关 键 词：潞玉13 自交系1572 干旱 基因表达 

分 类 号：S513[农业科学—作物学]