Apoptosis of colon cancer CT-26 cells induced polysaccharide from Cyclocarya paliurus and its phosphorylated derivative via intrinsic mitochondrial passway  

作　　者：Liuming Xie Mingyue Shen Rong Huang Xuan Liu Yue Yu Hanyu Lu Jianhua Xie 

机构地区：[1]State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China

出　　处：《Food Science and Human Wellness》2023年第5期1545-1556,共12页食品科学与人类健康（英文）

基　　金：supported by the National Natural Science Foundation of China (82060594);the Natural Science Foundation of Jiangxi province,China (20202BAB205006);The National Youth Talent Support Program of China。

摘　　要：In this study,the antitumor properties and the possible molecular mechanisms of Cyclocarya paliurus polysaccharide(CP)and its phosphorylated derivative(P-CP)on CT-26 mouse colon carcinoma cells were investigated.Results found that CP had high inhibition ratio against CT-26 cells.The flow cytometry results found that CP treatment could cause the intracellular acidification,arrest the cell cycle in the S phase and increase reactive oxygen species generation.Additionally,CP treatment triggered mitochondrial membrane potential depolarization and Ca^(2+)overloading,and broke down the balance of antioxidant system,Na^(+)/K^(+)-ATPase and Ca^(2+)-ATPase.Further analysis found CP induced cell apoptosis through improving the activities of caspase-3 and caspase-9,and increasing the level of cytochrome C.Furthermore,the comparative study of antitumor effect on CT-26 cells displayed that the phosphorylation enhanced antitumor activities of polysaccharides.These results suggest CP is a potential natural therapeutic agent for colon cancer and phosphorylation represents an effective method of enhancing the antitumor activity of CP.

关 键 词：Cyclocarya paliurus polysaccharide Phosphorylated Colorectal cancer Mitochondrial apoptosis pathway 

分 类 号：R285[医药卫生—中药学]