The dual-function of bioactive peptides derived from oyster (Crassostrea gigas) proteins hydrolysates  

作　　者：Dongyang Zhu Zhen Yuan Di Wu Chao Wu Hesham R.El-Seedi Ming Du 

机构地区：[1]School of Food Science and Technology,National Engineering Research Center of Seafood,Dalian Polytechnic University,Dalian 116034,China [2]Pharmacognosy Group,Department of Pharmaceutical Biosciences,Uppsala University,Biomedical Centre,Uppsala SE 75124,Sweden [3]International Research Center for Food Nutrition and Safety,Jiangsu University,Zhenjiang 212013,China

出　　处：《Food Science and Human Wellness》2023年第5期1609-1617,共9页食品科学与人类健康（英文）

基　　金：financially supported by the National Natural Science Foundation of China (32130085)。

摘　　要：Oysters(Crassostrea gigas)have a wide range of functionality due to their nutritional and bioactive components. However, the bioactive peptides of oyster proteins are rarely reported, particularly their antidiabetes effects and antioxidants. Oyster proteins were extracted from fresh oysters using phosphatebuffered saline and simulated gastrointestinal digestion was performed. The degree of hydrolysis(DH), structural characterization, molecular weight(Mw)distribution, free amino acid, anti-diabetic activity, and antioxidant activity were studied during in vitro simulated gastrointestinal digestion. The results showed that the α-glucosidase inhibitory activity, α-amylase inhibitory activity, DPPH radical scavenging activity, and ABTS radical scavenging activity of the oyster protein gastrointestinal digest were increased(P < 0.05)from 0 to 33.96%, from 9.17% to 44.22%, from 9.01 μg trolox/mg protein to 18.48 μg trolox/mg protein, and from 21.44 μg trolox/mg protein to 56.21 μg trolox/mg protein, respectively. Additionally, the DH, β-turn structure, fluorescence intensity, free amino acid, and short peptide content(Mw < 1 000 Da)increased in the simulated gastrointestinal digestion. These results indicate that the digestive hydrolysates obtained from oyster proteins could be used as natural anti-diabetic and antioxidant agents.

关 键 词：Oyster proteins Simulated gastrointestinal digestion Anti-diabetic activity Antioxidant activity Free amino acid 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]