Flammulina velutipes polysaccharide-iron (Ⅲ) complex used to treat iron deficiency anemia after being absorbed via GLUT2 and SGLT1 transporters  

作　　者：Chenying Shi Chen Cheng Xiaotong Lin Yanfang Qian Yufeng Du Guitang Chen 

机构地区：[1]Department of Food Nutrition and Safety/National R&D Center for Chinese Herbal Medicine Processing,China Pharmaceutical University,Nanjing 211198,China [2]College of Pharmacy,China Pharmaceutical University,Nanjing 211198,China

出　　处：《Food Science and Human Wellness》2023年第5期1828-1840,共13页食品科学与人类健康（英文）

基　　金：supported by the State key research and development plan “Modern food processing and food storage and transportation technology and equipment” (2017YFD0400203)。

摘　　要：Iron deficiency anemia(IDA)is a common nutritional problem, but traditional iron supplements cause many adverse reactions. Thus, the development of a novel iron supplement might be significant for the treatment of IDA. This study aimed to study the transport mechanism of Flammulina velutipes polysaccharide-iron complex(FVP1-Fe(Ⅲ))in Caco-2 cells and the therapeutic effect on IDA rats, as well as the influence on gut microbiota in vivo. These results showed that in vitro, the uptake of FVP1-Fe(Ⅲ)was mediated by sodium-dependent glucose transporter-1(SGLT1)and facilitated glucose transporter-2(GLUT2)and GLUT2 played a dominant function. The multidrug resistance-associated protein-2(MRP-2)was involved in the efflux of FVP1-Fe(Ⅲ)across the Caco-2 cells. In vivo, FVP1-Fe(Ⅲ)had a better restorative effect on blood parameters and iron status indicators in rats with IDA as compared with FeSO_4 and exerted this effect by downregulating the expression of hepcidin. FVP1-Fe(Ⅲ)could also regulate gut microbiota dysbiosis in iron deficiency rats by returning the relative abundance of gut microbiota to the normal level. Besides, as a dietary factor, vitamin C(vit C)could enhance the therapeutic effect of FVP1-Fe(Ⅲ). These present findings showed that FVP1-Fe(Ⅲ)could be exploited as a novel iron supplement to treat IDA.

关 键 词：Flammulina velutipes polysaccharide Iron deficiency anemia Iron supplement Caco-2 cell model Gut microbiota 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]