嘉兴市农田土壤中抗生素抗性基因的分布特征  

Distribution Characteristics of Antibiotic Resistance Genes in Farmland Soil of Jiaxing City

作　　者：吴英 张家萍 刘锐[3] WU Ying;ZHANG Jiaping;LIU Rui(Jiaxing Technician Institute,Jiaxing Zhejiang 314036,China;ZhongFa Holding GroupCo.LTD,Jiaxing Zhejiang 314008,China;Jiaxing Vocational Technical College,JiaxingZhejiang 314036,China)

机构地区：[1]嘉兴技师学院,浙江嘉兴314036 [2]中法控股集团有限公司,浙江嘉兴314008 [3]嘉兴职业技术学院,浙江嘉兴314036

出　　处：《北京农业职业学院学报》2023年第2期70-75,共6页Journal of Beijing Vocational College of Agriculture

基　　金：嘉兴市科技局项目(K2022C005)。

摘　　要：为了了解嘉兴市农田土壤中抗生素抗性基因分布现状,采用PCR技术对嘉兴市农田土壤中12种抗生素抗性基因进行了检测。结果表明,嘉兴市农田土壤样品中ermB、ermC、ermF、ermA、tetB、tetC、tetM、tetO、tetQ、tetW、sul1和sul2基因的检出率分别为65.63%、71.88%、100%、53.13%、46.88%、84.38%、90.63%、87.50%、84.38%、71.88%、93.75%和100%。检测结果可以为嘉兴市从抗生素抗性基因角度评估农艺措施提供指导依据,为嘉兴市掌握污染动态变化情况控制抗生素抗性基因传播提供基础理论数据。In order to understand the distribution of antibiotic resistance genes in farmland soil of Jiaxing City,12 antibiotic resistance genes in farmland soil of Jiaxing City were detected by PCR.The results showed that the detection rates of ermB,ermC,ermF,tetA,tetB,tetC,tetM,tetO,tetQ,tetW,sul1 and sul2 genes in Jiaxing farmland soil samples were 65.63%,71.88%,100%,53.13%,46.88%,84.38%,90.63%,87.50%,84.38%,71.88%,93.75%and 100%,respectively.The detection results provides guidance for Jiaxing to evaluate agronomic measures from the perspective of antibiotic resistance genes,and provide basic theoretical data for Jiaxing to master the dynamic changes of pollution and control the transmission of antibiotic resistance genes.

关 键 词：农田土壤 抗生素抗性基因 嘉兴市 

分 类 号：S154.1[农业科学—土壤学]