主要禾谷类作物DGAT基因家族比较分析  

Identification and Bioinformatics Analysis of DGAT Gene Family in Cereal Crops

作　　者：孟亚轩 姚旭航 孙颖琦 赵心月 王凤霞 瓮巧云[1] 刘颖慧[1] Meng Yaxuan;Yao Xuhang;Sun Yingqi;Zhao Xinyue;Wang Fengxia;Weng Qiaoyun;Liu Yinghui(College of Agriculture and Forestry Science and Technology,Hebei North University,Zhangjiakou 075000,Hebei,China)

机构地区：[1]河北北方学院农林科技学院,河北张家口075000

出　　处：《作物杂志》2023年第1期20-29,共10页Crops

基　　金：现代种业科技创新专项(21326302D);杂交谷子产业链创新(16226310D);河北省科技厅项目(19226420D)。

摘　　要：二酰甘油酰基转移酶(DGAT)是三酰甘油合成的限速酶,在植物油脂合成中发挥重要作用。为明确禾谷类作物DGAT基因家族的表达特征,利用已知DGAT成员序列对主要禾谷类作物进行全基因组扫描,分析其亚型差异及表达特点。结果表明,DGAT蛋白具有高脂肪、碱性和不稳定性。系统发育树中DGAT成员聚类为4个亚族,不同亚族成员间平行进化。DGAT基因结构具有组内保守性和组间多样性,基序存在多种类型,不同亚型成员具有特异的结构域组成。DGAT上游序列中,存在植株发育和胁迫响应等多种类型顺式作用元件,说明DGAT广泛参与不同生物学过程。转录组分析结果显示,DGAT广泛存在于不同组织中。在遭遇干旱和低温等不同非生物胁迫时,DGAT受到不同程度诱导,具有时空表达差异性。本研究可为禾谷类作物DGAT基因家族成员功能研究奠定基础。Diacylglycerol acyltransferase(DGAT)is a rate-limiting enzyme for triacylglycerol synthesis and plays an important role in plant oil synthesis.To clarify the expression characteristics of the DGAT gene family in cereal crops,this study performed a genome-wide scan of major cereal crops using known DGAT member sequences to analyze their isoform differences and expression characteristics.The results showed that DGAT proteins were highly fatty,basic and unstable.The DGAT members in the phylogenetic tree were clustered into four subclades,and the members of different subclades evolved in parallel with each other.The gene structure of DGATs was conserved within groups and diverse between groups,and there were multiple types of motifs with specific structural domain compositions of different isoforms.There were multiple types of cis-acting elements in the upstream sequences of DGATs,such as plant development and stress response,indicating that DGATs were widely involved in different biological processes.Transcriptome analysis showed that DGATs were widely present in different tissues.In response to different abiotic stresses such as drought and low temperature,DGATs were induced to different degrees with differential spatial and temporal expression.This study may lay the foundation for functional studies of DGAT gene family members in cereal crops.

关 键 词：禾谷类作物 DGAT基因家族 生物信息学分析 表达分析 

分 类 号：S51[农业科学—作物学]