消水散对人肠癌小鼠腹腔移植模型B7/CD28/CTLA-4协同刺激通路及腹水微环境的影响  

Effect of Xiaoshui powder on the B7/CD28/cytotoxic T lymphocyte-associated antigen 4 co-stimulation pathway and ascites microenvironment in a xenograft tumor mouse model of human colorectal cancer

作　　者：彭银花 彭巍[1] PENG Yinhua;PENG Wei(The Affiliated Hospital of Hunan Academy of Chinese Medicine/Hunan Provincial Hospital of Integrated Traditional Chinese and Western Medicine,Changsha 410006,Hunan,China)

机构地区：[1]湖南省中医药研究院附属医院/湖南省中西医结合医院,湖南长沙410006

出　　处：《湖南中医杂志》2023年第2期176-181,共6页Hunan Journal of Traditional Chinese Medicine

摘　　要：目的:研究消水散对人肠癌小鼠腹腔移植模型B7/CD28/细胞毒性T淋巴细胞相关抗原-4(CTLA-4)协同刺激通路及腹水微环境的影响。方法:采用腹腔注射人肠癌细胞株HCT116细胞建立人肠癌小鼠腹腔移植模型,分为模型组,消水散低、中、高剂量组,另设正常小鼠为对照组,每组各10只。观察阳虚症状积分,血清促肾上腺皮质激素(ACTH)、皮质醇(CORT)、三碘甲状腺原氨酸(T3)、甲状腺素(T4)、促甲状腺激素(TSH)含量,腹水量,瘤体积,瘤重,B7-1、B7-2、CD28、CTLA-4表达量及其mRNA表达水平,腹膜组织转化生长因子-β1(TGF-β1)、白细胞介素-10(IL-10)表达水平。结果:与正常组比较,模型组阳虚症状积分显著增加(P<0.01),ACTH、CORT、T3、T4、TSH含量显著降低(P<0.01)。与模型组比较,消水散呈剂量依赖性降低小鼠腹水量、瘤体积、瘤重、CTLA-4水平、TGF-β1、IL-10表达水平(P<0.05),增加B7-1、B7-2、CD28水平(P<0.05)。结论:消水散具有抗人肠癌移植瘤恶性腹水作用,这可能与抑制CTLA-4表达,促进T细胞激活,改善机体免疫抑制及腹腔免疫微环境有关。Objective: To investigate the effect of Xiaoshui powder on the B7/CD28/cytotoxic T lymphocyte-associated antigen 4(CTLA-4) co-stimulation pathway and ascites microenvironment in a xenograft tumor mouse model of human colorectal cancer.Methods: An abdominal xenograft tumor mouse model of human colorectal cancer was established by intraperitoneal injection of human colorectal cancer HCT116 cells, and then the mice were divided into model group and low-,middle-,and high-dose Xiaoshui powder groups, while normal mice were established as control group, with 10 mice in each group.The above groups were observed in terms of the following indicators: the symptom score of Yang deficiency;the content of adrenocorticotropic hormone(ACTH),corticosterone(CORT),triiodothyronine(T3),thyroxine(T4),and thyroid stimulating hormone(TSH) in serum;ascites volume;tumor volume and tumor weight;the protein and mRNA expression levels of B7-1,B7-2,CD28,and CTLA-4;the expression levels of transforming growth factor-beta 1(TGF-β1) and interleukin-10(IL-10) in peritoneal tissue.Results: Compared with the control group, the model group had a significant increase in the symptom score of Yang deficiency(P<0.01) and significant reductions in the content of ACTH,CORT,T3,T4,and TSH(P<0.01).Compared with the model group, Xiaoshui powder reduced ascites volume, tumor volume, tumor weight, CTLA-4 level, and the expression levels of TGF-β1 and IL-10(P<0.05) and increased the levels of B7-1,B7-2,and CD28(P<0.05) in a dose-dependent manner.Conclusion: Xiaoshui powder can exert a therapeutic effect on malignant ascites due to the xenograft tumor of human colorectal cancer, possibly by inhibiting the expression of CTLA-4,promoting T cell activation, and improving immunosuppression and abdominal immune microenvironment.

关 键 词：消水散 人肠癌小鼠腹腔移植模型 B7/CD28/细胞毒性T淋巴细胞相关抗原-4协同刺激通路 腹水微环境 

分 类 号：R285.5[医药卫生—中药学]