茄子青枯病病原菌的分离与鉴定  

Isolation and Identification of Pathogen of Eggplant Bacterial Wilt

作　　者：谢鑫鑫[1] 黄建都[1] 洪鹏翔[2] 陈继兵[1] 林翮飞[1] 陈仁[1] XIE Xin-xin;Huang Jian-dou;Hong Peng-xiang;Chen Ji-bing;Lin He-fei;Chen Ren(Fuzhou Institute of Vegetable Science,Fuzhou,Fujian 350111,China;College of Plant Protection,Fujian Agricultural and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福州市蔬菜科学研究所,福建福州350111 [2]福建农林大学植物保护学院,福建福州350002

出　　处：《东南园艺》2022年第6期408-413,共6页Southeast Horticulture

基　　金：福州市农业科技项目(2021-N-076);福建省种业项目(zycxny2021008)。

摘　　要：为鉴定福州地区茄子青枯病病原菌,本试验从田间疑似患青枯病的茄子植株中进行病原菌的分离和鉴定,该病原菌的形态、发病症状与青枯病病原菌的形态和发病症状相符。再通过对病原菌的16S rDNA序列进行PCR扩增,测序分析比对后发现该病原菌菌株与茄科青枯雷尔氏菌的同源性为99.79%。重新接种健壮植株,再次从患病组织分离病原菌,发现分离的病原菌其形态及16S rDNA序列与之前接种的病原菌一致。说明本研究分离出了茄子青枯病病原菌,该病原菌为青枯劳尔氏菌,能够使植株表现出青枯病症状,病原菌的分离为茄果类蔬菜青枯病的防治及抗性育种奠定了基础。In order to identify the pathogen of eggplant bacterial wilt in Fuzhou area,this experiment was conducted to isolate and identify the pathogen from the eggplant plants suffering from bacterial wilt in the field. The results showed that the morphology and symptoms of the pathogen were consistent with those of Ralstonia solanacearum. The 16S rDNA sequence of the pathogen was amplified by polymerase chain reaction(PCR). The sequencing alignment showed that the homology between the pathogen strain and Raleira solanacearum was 99.79%. Healthy plants were inoculated and the pathogen was isolated from the diseased tissues again. It was found that the morphology and 16S rDNA sequence of the isolated pathogens were identical to those of the previously inoculated pathogens. It showed that the pathogen of eggplant bacterial wilt was isolated in this study,and the pathogen was Lauersolanum solanum,which could make the plants show the symptoms of bacterial wilt. The isolation of the pathogen laid the foundation for the control of bacterial wilt and resistance breeding of vegetables.

关 键 词：茄子 青枯病 16S rDNA 致病性 

分 类 号：S634.3[农业科学—蔬菜学]