小鼠无线头皮脑电图技术平台的建立及其在睡眠研究中的应用与验证  

Establishment of wireless scalp electroencephalogram technology platform in mice and its application and verification in sleep research

作　　者：孙传耀 刘爽[1] 张树卓 苏瑞斌 SUN Chuan-yao;LIU Shuang;ZHANG Shu-zhuo;SU Rui-bin(College of Basic Medicine Jiamusi University,Jiamusi 154000,China;Beijing Key Laboratory of Neuropsychopharmacology,State Key Laboratory of Toxicology and Medical Countermeasures,Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China)

机构地区：[1]佳木斯大学基础医学院,黑龙江佳木斯154000 [2]军事科学院军事医学研究院毒物药物研究所,抗毒药物与毒理学国家重点实验室,神经精神药理学北京市重点实验室,北京100850

出　　处：《中国药理学与毒理学杂志》2023年第1期41-46,共6页Chinese Journal of Pharmacology and Toxicology

基　　金：黑龙江省教育厅基础研究项目(2019-KYYWF-1336)。

摘　　要：目的 建立小鼠无线头皮脑电图(EEG)记录平台。方法 小鼠颅骨表面植入EEG/肌电图(EMG)底座,术后恢复7 d开始实验。分别在第1天18∶00 ip给予溶剂(1%DMSO)和第2天同一时间ip给予镇静催眠药地西泮(DZP)1.0 mg·kg^(-1),随后采用Pinnacle EEG/EMG无线记录系统采集自由活动小鼠ip注射后3 h内EEG和EMG数据,最后使用睡眠分析软件Sirenia^(■)Sleep pro分析EEG和EMG信号,判读睡眠-觉醒时相,通过MATLAB软件分析滤波后数据的功率谱。结果 该平台可高效且准确地采集和解析小鼠睡眠-觉醒行为。与溶剂组相比,DZP 1.0 mg·kg^(-1)增加非快眼动睡眠和总睡眠时间,减少觉醒时间(P<0.05);同时,降低睡眠期δ和θ波功率谱(P<0.05,P<0.01),增加睡眠期慢波振荡-睡眠纺锤波相位-振幅耦合度(P<0.05)。结论 成功建立小鼠无线头皮EEG技术平台,此平台可用于研究睡眠障碍发病机制及筛选新药。OBJECTIVE To establish a wireless scalp electroencephalogram (EEG) recording platform in mice.METHODS An EEG/electromyogram (EMG) electrode was implanted on the surface of the skull of mice.The experiment was conducted after a week of sursery.The vehicle (1%DMSO) was ip given at 17∶55 on the 1stday and diazepam (DZP) 1.0 mg kg-1was ip given at the same time on the 2ndday before the Pinnacle EEG/EMG wireless recording system was used to collect the scalp EEG and EMG data of free-moving mice within 3 h of treatment.Finally,sleep analysis software Sirenia^(■)Sleep pro was used to analyze EEG and EMG,interpret the sleep-awakening phase,and analyze the power spectrum of filtered data by MATLAB software.RESULTS The platform could efficiently and accurately record and analyze the sleep-awakening behavior of mice.The wireless EEG could efficiently record and interpret the sleep-awakening phase.Compared with the solvent group,DZP 1.0 mg·kg^(-1)increased non-rapid eye movement and total sleep time and decreased awakening time (P<0.05),while DZP 1.0 mg·kg^(-1)decreased the power spectrum of δ and θ waves during sleep (P<0.05,P<0.01),and increased the phase-amplitude coupling of slow oscillation-spindle during sleep (P<0.05) compared with the vehicle group.CONCLUSION A platform for sleep wireless scalp EEG in mice has been established,which can be used to explore the pathogenesis of sleep disorders and screen new drugs.

关 键 词：无线头皮脑电图技术平台 睡眠 地西泮 脑电图 肌电图 

分 类 号：R965.2[医药卫生—药理学]