七叶皂苷钠对氧化型低密度脂蛋白诱导的血管平滑肌细胞增殖和迁移的影响  

Effects of sodium aescinate on proliferation and migration of oxidized low-density lipoprotein induced vascular smooth muscle cells

作　　者：官泽宇[1] 宋涛[1] 余朝文[1] 陈世远[1] 徐超[1] GUAN Ze-yu;SONG Tao;YU Chao-wen;CHEN Shi-yuan;XU Chao(Department of Vascular Surgery,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233004,Anhui Province,China)

机构地区：[1]蚌埠医学院第一附属医院血管外科,安徽蚌埠233004

出　　处：《中国临床药理学杂志》2023年第5期639-643,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究七叶皂苷钠(SA)对氧化型低密度脂蛋白(ox-LDL)诱导的血管平滑肌细胞增殖和迁移的影响及其机制。方法将体外培养的人主动脉血管平滑肌细胞(HA-VSMC)随机分为对照组(正常培养不做处理)、模型组(以100μg·mL^(-1)ox-LDL处理24 h)和低、中、高剂量实验组(在ox-LDL处理的基础上分别以0.4,1.6和6.4μg·mL^(-1)的SA处理24 h)。用噻唑蓝(MTT)实验检测细胞增殖,以划痕愈合实验检测细胞迁移,以酶联免疫吸附(ELISA)法检测肿瘤坏死因子α(TNF-α)和超氧化物歧化酶(SOD)水平,用蛋白质印迹法检测蛋白激酶B(Akt)/核因子-κB(NF-κB)信号通路相关蛋白表达。结果对照组、模型组和低、中、高剂量实验组细胞OD450 nm值分别为0.31±0.03、0.90±0.06、0.76±0.06、0.54±0.04、0.36±0.02,迁移率分别为(56.36±3.38)%、(87.02±6.58)%、(78.74±4.55)%、(69.98±3.48)%、(62.68±4.02)%,TNF-α水平分别为(34.59±3.14)、(85.13±5.13)、(74.03±5.47)、(59.40±4.41)、(48.98±3.02)ng·L^(-1),SOD水平分别为(46.52±3.07)、(19.98±1.02)、(26.33±2.19)、(32.30±2.96)、(42.78±3.37)ng·L^(-1),p-Akt/Akt水平分别为0.18±0.02、0.87±0.05、0.73±0.06、0.50±0.04、0.32±0.03,p-p65/p65水平分别为0.12±0.02、0.79±0.05、0.66±0.04、0.45±0.03、0.27±0.03,p-IκBα/IκBα水平分别为0.22±0.03、0.92±0.07、0.80±0.06、0.68±0.03、0.41±0.03;模型组与对照组比较,低、中、高剂量实验组与模型组比较,差异均有统计学意义(均P<0.05)。结论SA可抑制ox-LDL诱导的血管平滑肌细胞增殖和迁移,其作用机制可能与抑制Akt/NF-κB信号通路介导的炎症和氧化应激有关。Objective To investigate the effects of sodium aescinate(SA)on the proliferation and migration of vascular smooth muscle cells induced by oxidized low-density lipoprotein(ox-LDL)and its mechanism.Methods Human aortic vascular smooth muscle cells(HA-VSMC)cultured in vitro were randomly divided into control group(normal culture without treatment),model group(100μg·m L^(-1)oxLDL treatment for 24 h)and low,medium and high dose experimental groupss(treated with 0.4,1.6 and 6.4μg·m L^(-1)SA for 24 h on the basis of ox-LDL treatment).Cell proliferation was detected by thiazolyl blue(MTT)assay and cell migration was detected by wound healing assay.The levels of tumor necrosis factorα(TNF-α)and superoxide dismutase(SOD)were detected by enzymelinked immunosorbent assay(ELISA),and the expression of protein kinase B(Akt)/nuclear factor-κB(NF-κB)signaling pathway related proteins was detected by Western blot.Results The OD450 nmvalues of control group,model group,and low,medium,high dose experimental groups were 0.31±0.03,0.90±0.06,0.76±0.06,0.54±0.04and 0.36±0.02;the migration rates were(56.36±3.38)%,(87.02±6.58)%,(78.74±4.55)%,(69.98±3.48)%,(62.68±4.02)%;TNF-αlevels were(34.59±3.14),(85.13±5.13),(74.03±5.47),(59.40±4.41),(48.98±3.02)ng·L^(-1);SOD levels were(46.52±3.07),(19.98±1.02),(26.33±2.19),(32.30±2.96),(42.78±3.37)ng·L^(-1);p-Akt/Akt levels were 0.18±0.02,0.87±0.05,0.73±0.06,0.50±0.04,0.32±0.03;p-p65/p65 levels were 0.12±0.02,0.79±0.05,0.66±0.04,0.45±0.03,0.27±0.03;p-IκBα/IκBαlevel were 0.22±0.03,0.92±0.07,0.80±0.06,0.68±0.03,0.41±0.03;there were statistically significant differences between model group and control group,between low,medium,high dose experimental groups and model group(all P<0.05).Conclusion SA can inhibit ox-LDL-induced proliferation and migration of vascular smooth muscle cells,which may be related to inhibition of inflammation and oxidative stress mediated by Akt/NF-κB signaling pathway.

关 键 词：氧化型低密度脂蛋白 血管平滑肌细胞 七叶皂苷钠 增殖 迁移 蛋白激酶B/核因子-κB信号通路 

分 类 号：R28[医药卫生—中药学]