Rapid detection of tyrosinase in shrimp by polyacid-based electrochemical sensors  

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作  者:Xiao-Wen Chen Yu-Shan Chen Xiao-Mei Ding Li Wang Jing Liu Rui-Fang Wang 

机构地区:[1]College of Food and Biological Engineering,Jimei University,Xiamen 361021,China [2]School of Information Engineering,Jimei University,Xiamen 361021,China

出  处:《Tungsten》2023年第1期81-90,共10页钨科技(英文)

基  金:the support of the National Natural Science Foundation of China (Grant No. 21871110);the Natural Science Foundation of China Fujian Province (Grant No. 2020J01674)。

摘  要:The increase through tyrosinase levels after shrimp death causes enzymatic browning,affecting the appearance and freshness of products,and it is essential to establish a simple and rapid method to detect tyrosinase in the aquatic product supply chain to control the market’s good quality.In this study,we used a constant potential deposition method to create a [(Polystyrene Sodium Sulfonate(PS S)/Polypyrrole(PPy))(P2Mo17Ni/PPy)5] polystyrene sodium sulfonate polymetallic oxide thin film electrodes that can realize the detection of tyrosinase(TYR) in shrimp samples.The method yielded a limit of detection(Signal-Noise ratio(S/N)=3) of 0.1028 U·mL^(-1) within the linear detection of 3.22-26.04 U·mL^(-1),and the recoveries of the samples ranged from 96.37% to 102.40%.The above data confirmed that the method showed some accuracy in the detection of TYR content in shrimps and provided a theoretical basis for the preparation of tyrosinase sensors for the application of polyoxometalates in aquatic products.

关 键 词:POLYOXOMETALATES Electrochemical TYROSINASE Sensors 

分 类 号:TP212[自动化与计算机技术—检测技术与自动化装置]

 

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