2018—2019年济南市人鼻病毒和呼吸道合胞病毒及腺病毒感染分析  

Epidemiological analysis of human rhinovirus, respiratory syncytial virus and human adenovirus in Jinan from 2018 to 2019

作　　者：何玉洁 张淑 吕燕[2] 孙林[1] 吴巨龙[1] 宋绍霞[1] 孙文魁[1] 张曼 李忠[1] 赵怀龙[2] 寇增强[1] 刘倜[1] He Yujie;Zhang Shu;Lyu Yan;Sun Lin;Wu Julong;Song Shaoxia;Sun Wenkui;Zhang Man;Li Zhong;Zhao Huailong;Kou Zengqiang;Liu Ti(Institute of Infectious Disease Prevention and Control,Shandong Provincial Center for Disease Control and Prevention,Jinan 250014,China;Institute of Virology,Jinan Center for Disease Control and Prevention,Jinan 250021,China)

机构地区：[1]山东省疾病预防控制中心传染病防制所,济南250014 [2]济南市疾病预防控制中心病毒性疾病检验所,250021

出　　处：《中华实验和临床病毒学杂志》2023年第1期30-38,共9页Chinese Journal of Experimental and Clinical Virology

基　　金：山东省自然科学基金(ZR2021MH372);山东省医药卫生科技发展计划(202012051365)。

摘　　要：目的研究2018年4月至2019年3月济南市急性呼吸道感染病例中人鼻病毒、呼吸道合胞病毒及人腺病毒的感染情况及流行病学特点, 并对分离出的人腺病毒做全基因组序列分析。方法采集2018年4月至2019年3月济南市第四人民医院、山东大学齐鲁儿童医院、章丘区人民医院的急性呼吸道感染住院病例患者的鼻/咽拭子样本共计1 969份, 采用实时荧光定量PCR的方法检测样本中呼吸道相关病原体, 包括人鼻病毒、呼吸道合胞病毒及人腺病毒的阳性率, 并对分离出的7株腺病毒阳性标本进行测序, 以确定腺病毒型别, 并构建基因进化树进行分析。结果在收集的1 969份标本中, 3种呼吸道病毒阳性病例共计242例, 阳性检测率为12.30 %(242/1969), 鼻病毒阳性率为3.00%(59/1969)、呼吸道合胞病毒阳性率为6.30 %(124/1969)、腺病毒阳性率为3.86%(76/1969)。不同年龄段间鼻病毒检出率差异无统计学意义(Fisher精确检验值8.376, P=0.720), 不同年龄段间呼吸道合胞病毒、腺病毒检出率差异均有统计学意义(χ^(2)=19.28、12.16;P=0.001、0.016)。不同性别间腺病毒检出率差异具有统计学意义(χ^(2)=14.33, P<0.001), 不同性别间鼻病毒阳性率和呼吸道合胞病毒阳性率均无统计学意义(χ^(2)=0.30、2.90, P=0.862、0.089)。比较不同月份间三种病毒核酸阳性率, 发现鼻病毒、呼吸道合胞病毒和腺病毒的检出率分别在10月份、12月份、11月份达到最高(分别为8.61%、26.50%和8.84%)。对测序的7株腺病毒阳性标本构建基因进化树后发现其均为HAdV-2型。结论 2018年4月至2019年3月济南市急性呼吸道感染相关的人鼻病毒、呼吸道合胞病毒及人腺病毒在不同组别间有不同的流行病学特点, 为急性呼吸道感染的早期预防和临床治疗提供参考依据。Objective To study the infection of human rhinovirus,respiratory syncytial virus and human adenovirus in Jinan from April 2018 to March 2019,and analyze epidemiological characteristics of human adenovirus.Methods All of 1969 nasopharyngeal swabs were collected from hospitalized patients with acute respiratory tract infections in The Fourth People’s Hospital of Jinan,Qilu Children′s Hospital of Shandong University,Peoples Hospital of Zhangqiu District from April 2018 to March 2019,fluorescence quantitative PCR was used to detect the positive rate of human rhinovirus,respiratory syncytial virus and human adenovirus.Seven adenovirus positive samples were isolated and examined by sequencing,then we determined adenovirus type,constructed gene phylogenetic trees for analysis.Results Of the 1969 samples,242 were positive,the total positive rate was 12.30%(242/1969),the positive rate was 3.00%(59/1969)for rhinovirus,6.30%(124/1969)for respiratory syncytial virus(RSV),and 3.86%(76/1969)for adenovirus.There was no significant difference in the detective rate of rhinovirus in different age groups(Fisher′s exact test value=8.376,P=0.720),the positive rates of RSV and adenovirus in different age groups was statistically significant(χ^(2)=19.28,12.16;P=0.001,0.016).There was a statistically significant difference in the positive rate of adenovirus between different sexes(χ^(2)=14.33,P<0.001),and there was no statistically significant difference in the positive rate of rhinovirus and RSV between males and females(χ^(2)=0.30,2.90,P=0.862,0.089).Comparing the positive rates of viral nucleic acid in different months,we found that the positive rate of rhinovirus,RSV and adenovirus separately reached the highest in October,December and November(8.61%,26.50%and 8.84%).We constructed a gene phylogenetic tree after seven positive samples of adenoviruses were sequenced,by the molecular typing method we detected that seven adenovirus-positive samples were all HAdV-2 type.Conclusions By comparing the epidemiological trends of hum

关 键 词：呼吸道相关病毒 流行特征 全基因组 基因分型 

分 类 号：R511[医药卫生—内科学] R181.3[医药卫生—临床医学]