可培养肠道细菌对南亚果实蝇生殖力的影响  

Effect of culturable intestinal bacteria on fecundity of Zeugodacus tau(Walker)

作　　者：丁鹏 刘崇敏 孙作恒 陈稳 沈翰韬 张宏宇[1] 张振宇 袁伊旻 DING Peng;LIU Chongmin;SUN Zuoheng;CHEN Wen;SHEN Hantao;ZHANG Hongyu;ZHANG Zhenyu;YUAN Yimin(College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;School of Environmental Design,Wuhan Design and Engineering College,Wuhan 430205,China)

机构地区：[1]华中农业大学植物科学技术学院,武汉430070 [2]武汉设计工程学院环境设计学院,武汉430205

出　　处：《华中农业大学学报》2023年第2期115-122,共8页Journal of Huazhong Agricultural University

基　　金：国家重点研发计划项目(2021YFD1400803);湖北省教育厅科研计划项目(B2019329)。

摘　　要：为明确检疫实蝇害虫南亚果实蝇(Zeugodacus tau Walker)雌成虫的肠道优势菌株种类及它们对南亚果实蝇取食能力、营养状态和生殖力的影响,通过平板分离与分子鉴定技术进行可培养菌株的分离与菌种分析,进一步进行抗生素处理与肠道优势菌株回补处理,检测南亚果实蝇取食量、血淋巴氨基酸含量和产卵量。结果显示:清除肠道细菌可显著降低南亚果实蝇雌成虫的产卵量、取食量和血淋巴氨基酸含量,降至正常雌成虫(无抗生素处理,对照)水平的34.9%、43.2%和60.8%。鉴定出的9种可培养菌株,分别隶属于变形菌门(Proteobacteria)和厚壁菌门(Firmicutes),主要分布在肠球菌属(Enterococcus)、肠杆菌属(Enterobacter)和芽孢杆菌属(Bacillus);而其中的优势菌株为Enterococcus sp. ZYY 2-97(57.41%)和Bacillus sp. ZYY 1-83(17.59%)。回补ZYY 2-97和ZYY 1-83菌株后南亚果实蝇雌成虫的总产卵量分别显著恢复至对照的75.00%和86.98%,取食量显著恢复至对照水平的117.53%和117.85%,而血淋巴氨基酸含量显著恢复至对照水平的94.80%和87.81%。以上结果表明,肠道细菌可通过调控南亚果实蝇雌成虫的取食能力和营养状态而影响宿主生殖力,而且其中的优势菌株发挥了关键作用。To identify the dominant strains in the intestinal tract in female adults of the quarantine fruit fly pest,Zeugodacus tau(Walker),and their effects on feeding ability,nutritional status and fecundi⁃ty,here traditional microorganism isolation and molecular identification techniques were use to isolate and identify the culturable bacteria,and then antibiotic treatment and intestinal dominant strains supplementa⁃tion were carried out to detect the intake,hemolymph amino acid content and egg production of Z.tau.The results indicated that the elimination of intestinal bacteria significantly reduced the oviposition,food intake and hemolymph amino acid content of female adult fruit flies to 34.9%,43.2% and 60.8% of the control(without antibiotic treatment)levels.Subsequently,9 cultivable strains were isolated and identified as Pro⁃teobacteria and Firmicutes,and are mainly distributed in the genera of Enterococcus,Enterobacter and Ba⁃cillus.The dominant strains were Enterococcus sp.ZYY 2-97(57.41%)and Bacillus sp.ZYY 1-83(17.59%).In addition,after supplemented with ZYY 2-97 and ZYY 1-83 strains,the total fecundity of the female adults were significantly recovered to 75.00% and 86.98% of the control level,and the food in⁃take were significantly recovered to 117.53% and 117.85% of the control level,respectively.Meanwhile,the amino acids contents in hemolymph were recovered to 94.80% and 87.81% of the control level.Our findings demonstrated that the intestinal bacteria could affect the fecundity of Z.tau by regulating the feed⁃ing capacity and nutritional status of the female adults,and the dominant culturable strains play a key role in this process.

关 键 词：南亚果实蝇 肠道微生物 可培养菌 生殖力 取食能力 营养状态 绿色防控 

分 类 号：S433[农业科学—农业昆虫与害虫防治]