云南省某中学一起诺如病毒暴发疫情的病原鉴定和基因特征分析  

Pathogen identification and genetic characteristics of a norovirus outbreak in a middle school in Yunnan Province

作　　者：李楠 姜黎黎[1] 周永明[1] 牟建春 倪刚 周晓芳[1] 曹亿会[1] Li Nan;Jiang Lili;Zhou Yongming;Mou Jianchun;Ni Gang;Zhou Xiaofang;Cao Yihui(Institute for Acute Communicable Disease Prevention and Control,Yunnan Center for Disease Control&Prevention,Kunming 650022,China;Division for Prevention and Control of Acute Infectious Diseases and Emergency Handling of Public Health Emergencies,Honghe Center for Disease Control and Prevention,Mengzi 661199,China;Health Laboratory Center,Honghe Center for Disease Control and Prevention,Mengzi 661199,China)

机构地区：[1]云南省疾病预防控制中心急性传染病防制所,昆明650022 [2]云南省红河州疾病预防控制中心急性传染病防制与突发公共卫生事件应急处置科,蒙自661199 [3]云南省红河州疾病预防控制中心卫生检验中心,蒙自661199

出　　处：《国际流行病学传染病学杂志》2023年第1期63-67,共5页International Journal of Epidemiology and Infectious Disease

基　　金：云南省重大传染病疫苗研发重点实验室开放研究基金 (2020KF001)。

摘　　要：目的明确2022年云南省某中学一起急性肠胃炎暴发疫情的病原和基因特征。方法采集这起暴发疫情中的学生和厨工病例的肛拭子,使用诺如病毒GⅠ/GⅡ双通道核酸检测试剂盒定性检测。GⅠ和GⅡ阳性的样本分别采用引物G1SKF/G1SKR和MON431/G2SKR进行病毒扩增和测序,通过在线分型工具及系统进化分析确定病毒基因型别。结果共采集58份肛拭子,实验室检测阳性率为53.45%(31/58)。其中,GⅠ阳性样本22份,GⅡ阳性样本14份,GⅠ和GⅡ合并感染样本5份。31份阳性样本基因测序获得毒株序列22株,包括诺如病毒GⅠ型15株,其中GⅠ.6[P6]型9株、GⅠ6.[P11]型6株;诺如病毒GⅡ.4[P16]型7株。结论本起疫情由诺如病毒GⅠ.6[P6]型、GⅠ.6[P11]型和GⅡ.4[P16]型混合感染引起,这是云南省首次在诺如病毒暴发疫情中检出GⅠ型。Objective To clarify the pathogen and genetic characteristics of an outbreak of acute gastroenteritis in a middle school in Yunnan Province in 2022.Methods Rectal swabs collected from students and kitchen workers in this outbreak were detected by norovirus GⅠ/GⅡnucleic acid detection kit.The aimed part of norovirus from GⅠ/GⅡ-positive samples were amplified and sequenced by primer G1SKF/G1SKR and MON431/G2SKR.The genotypes were preliminarily determined by online typing tools and phylogenetic analysis.Results A total of 58 rectal swabs were tested,of which 31 samples were positive with the positive rate of 53.45%(31/58).Among them,22 samples were positive for GⅠand 14 samples were positive for GⅡ.There were 5 samples co-infected with GⅠand GⅡ.A total of 22 sequences were obtained from 31 positive samples.There were 15 GⅠstrains including 9 GⅠ.6[P6]strains and 6 GⅠ.6[P11]strains,and 7 GⅡ.4[P16]strains.Conclusions This outbreak is caused by norovirus GⅠ.6[P6],GⅠ.6[P11]and GⅡ.4[P16].It is the first time that GⅠgenotype is detected in the norovirus outbreak in Yunnan Province.

关 键 词：诺罗病毒 急性肠胃炎 暴发 GⅠ型 

分 类 号：R725.1[医药卫生—儿科] R181.8[医药卫生—临床医学]