羟腈裂解酶在合成化学中应用的研究进展  

Recent progress in hydroxynitrile lyases:utilization in the synthetic chemistry

作　　者：郑宇璁 郁惠蕾 许建和[1] Yu-Cong Zheng;Hui-Lei Yu;Jian-He Xu(State Key Laboratory of Bioreactor Engineering,Shanghai Collaborative Innovation Center for Biomanufacturing,School of Biotechnology,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]华东理工大学生物工程学院,反应器工程国家重点实验室,上海生物制造产业协同创新中心,上海200237

出　　处：《中国科学：化学》2023年第3期300-311,共12页SCIENTIA SINICA Chimica

基　　金：国家重点基础研究发展规划(编号:2019YFA0905000);国家自然科学基金(编号:21922804);中国科学院上海有机化学所开放课题资助项目。

摘　　要：作为少数可以用于不对称催化C−C键合成/裂解的几类酶之一,羟腈裂解酶因其可在相对温和的条件下实现氰醇或β-硝基醇的高对映体选择性合成而受到了合成化学家的青睐.有赖于分子生物学手段和蛋白质工程的快速发展,近年来羟腈裂解酶在不对称合成中的应用得到进一步扩展.本文就近年来耦合蛋白质工程改造后的羟腈裂解酶催化不对称羟氰化和硝基-羟醛缩合在生物活性分子的合成中的代表性应用进行总结,并展望了未来羟腈裂解酶的发展方向.As one of the few biocatalysts which can catalyze asymmetric C–C bond formation,hydroxynitrile lyases have emerged as attractive biocatalysts in terms of their high chemo-and enantioselectivity for the synthesis cyanohydrins andβ-nitro alcohols under mild conditions.With the enabling technology on molecular biology and protein engineering,the application of hydroxynitrile lyases in asymmetric synthesis has been greatly expanded.We herein summarized the basic traits and principles of biocatalytic asymmetric hydrocyanation and nitroaldol reactions.In addition,the representative examples of recent biocatalytic asymmetric synthesis of bioactive compounds via hydrocyanation or nitroaldol reaction as key steps are exemplified,and the future directions are briefly discussed.

关 键 词：羟腈裂解酶 手性氰醇 不对称催化 非天然底物 蛋白质工程 

分 类 号：O621.3[理学—有机化学] TQ426.97[理学—化学]