双香豆素对猪繁殖与呼吸综合征病毒的体外抑制作用  

Inhibitory Effect of Dicumarol on Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus in vitro

作　　者：李倬伟 王方 王君君 陈祯涵 李焕荣[1] 周双海[1] 刘雪威 LI Zhuowei;WANG Fang;WANG Junjun;CHEN Zhenhan;LI Huanrong;ZHOU Shuanghai;LIU Xuewei(College of Animal Science and Technology,Beijing University of Agriculture,Beijing 100096,China)

机构地区：[1]北京农学院动物科学技术学院,北京100096

出　　处：《畜牧兽医学报》2023年第3期1160-1168,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金青年科学基金项目(32102663);北京市教育委员会科学研究计划项目(KM202210020005);北京农学院青年科学基金(QNKJ202106)。

摘　　要：为探究双香豆素(DIC)对猪繁殖与呼吸综合征病毒(PRRSV)增殖的影响,本研究首先通过CCK-8法检测DIC对Marc-145细胞的细胞毒性,并计算其半数毒性浓度(CC_(50));进而使用荧光定量PCR和Western blot检测DIC对PRRSV增殖水平的影响;通过不同给药方式处理细胞进一步探究DIC针对的PRRSV增殖阶段。结果表明,DIC对Marc-145细胞的细胞毒性CC_(50)为96.11μmol·L^(-1),并在浓度为25μmol·L^(-1)时即表现出明显的抗PRRSV活性;DIC可以呈剂量依赖性地抑制不同时间和不同滴度的PRRSV增殖。DIC对PRRSV感染周期的吸附、入胞和释放阶段无影响,但是可明显抑制PRRSV的复制阶段。本研究证实DIC以较低浓度在Marc-145细胞中表现出明显的抗PRRSV活性,并主要针对PRRSV的复制阶段。本研究提示双香豆素具备开发成临床抗病毒药物或添加剂的潜力,为新的PRRSV抗病毒药物的研发提供理论依据。In this study,we investigated the effect of dicumarol(DIC)on the proliferation of porcine reproductive and respiratory syndrome virus(PRRSV).Firstly,the cytotoxicity of DIC on Marc-145 cells was detected by CCK-8 method and calculated the half toxicity concentration(CC_(50)).Then,the effect of DIC on the proliferation of PRRSV was detected using qPCR and Western blot.Further,DIC targeted stage of PRRSV proliferation were explored by treating the cells with different administration methods.The results showed that the CC_(50)of DIC against Marc-145 cells was 96.11μmol·L^(-1)and DIC exhibited significant anti-PRRSV activity at a concentration of 25μmol·L^(-1);DIC inhibited PRRSV proliferation in a dose-dependent manner at different times and titers.DIC had no effect on the adsorption,entry and release of the PRRSV infection cycle but significantly inhibited the replication of PRRSV.This study confirmed that DIC exhibited significant anti-PRRSV activity in Marc-145 cells at low concentrations and mainly targeted the replication phase of PRRSV,suggesting that DIC have the potential to be developed into clinical antiviral drugs or additives,providing a theoretical basis for the development of new PRRSV antiviral drugs.

关 键 词：双香豆素 猪繁殖与呼吸综合征病毒 PRRSV 抗病毒 

分 类 号：S852.659.6[农业科学—基础兽医学]