猪产业链中鼠伤寒沙门菌及沙门菌血清型4,[5],12:i:-基因组学分析  

Genomic Analysis of Salmonella Typhimurium Isolates and Salmonella Serotype 4,[5],12:i:-Isolates from Pig-borne Food Chain

作　　者：蒋增海[1,3] 滕霖 贺安文 刘言言 乐敏 何启盖[3] JIANG Zenghai;TENG Lin;HE Anwen;LIU Yanyan;YUE Min;HE Qigai(College of Veterinary Medicine,Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China;Institute of Preventive Veterinary Medicine&Department of Veterinary Medicine,College of Animal Sciences,Zhejiang University,Hangzhou 310058,China;College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]河南牧业经济学院动物医药学院,郑州450046 [2]浙江大学动物科学学院动物医学系&动物预防医学研究所,杭州310058 [3]华中农业大学动物医学院,武汉430070

出　　处：《畜牧兽医学报》2023年第3期1199-1209,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：河南省科技攻关(212102110096);浙江省重点研发计划(2020C02032;2021C02008);财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-35);河南牧业经济学院博士启动基金(2022HNUAHEDF027)。

摘　　要：旨在探究鼠伤寒沙门菌及沙门菌血清型4,[5],12:i:-基因组学特征。采用Illumina NovaSeq 6000平台对91株河南省猪产业链中鼠伤寒沙门菌及沙门菌血清型4,[5],12:i:-分离菌进行全基因组测序,通过生物信息学分析,进行其血清型的预测、多位点序列的分型以及质粒类型、耐药基因和毒力基因分析。结果表明,在河南省猪产业链中沙门菌4,[5],12:i:-的流行率(1.58%,50/3173)已经超过鼠伤寒沙门菌的流行率(1.29%,41/3173);91株沙门菌的序列型分为ST34(71.42%,65/91)和ST19(28.57%,26/91);鼠伤寒沙门菌/ST34菌株和4,[5],12:i:-/ST34菌株都是IncHI2A_1、IncHI2_1和Col440I_1质粒的偏好宿主;沙门菌血清型4,[5],12:i:-携带率较高的耐药基因是blaTEM-1B_1(72%)、tet(B)_2(90%)、sul2_3(66%)、blaOXA-1_1(34%)等;相比鼠伤寒沙门菌菌株,沙门菌血清型4,[5],12:i:-菌株中质粒的携带率更高,且携带更多种类的耐药基因和毒力基因。本研究首次证实,河南省猪产业链中沙门菌血清型4,[5],12:i:-的流行已经超过鼠伤寒沙门菌,在一定程度上解释了该血清型在河南省病人中主要流行的原因。Whole genomes of 91 Salmonella isolates from pig-borne food chain of Henan province were sequenced by Illumina NovaSeq 6000 platform for the understanding of genomic characteristics of Salmonella typhimurium and Salmonella serotype 4,[5],12:i:-.Serotype prediction,multi-locus sequence typing,plasmid types,virulence genes and drug resistance genes were analyzed by bioinformatics.The results showed that the prevalence of Salmonella 4,[5],12:i:-(1.58%,50/3173)was higher than that of Salmonella typhimurium(1.29%,41/3173)in pig-borne food chain of Henan Province.Sequence types(STs)of 91 Salmonella isolates included ST34(71.42%,65/91)and ST19(28.57%,26/91).Typhimurium/ST34 strains and 4,[5],12:i:-/ST34 strains were the preferred hosts of some plasmids including IncHI2A_1,IncHI2_1 and Col440I_1.Resistant genes highly carried by Salmonella serotypes 4,[5],12:i:-were blaTEM-1B_1(72%),tet(B)_2(90%),sul2_3(66%),blaOXA-1_1(34%),etc.Compared with Salmonella typhimurium isolates,Salmonella serotype 4,[5],12:i:-isolates had a higher plasmid-carrying rate,carrying a greater variety of resistance genes and virulence genes.This study confirmed for the first time that the prevalence of Salmonella serotype 4,[5],12:i:-has exceeded that of Salmonella typhimurium in pig-borne food chain of Henan Province.To a certain extent,this may explain the predominant prevalence of the Salmonella serotype among patients in the area.

关 键 词：沙门菌血清型4 [5] 12:i:- 鼠伤寒沙门菌单相变种 猪产业链 基因组学 耐药基因 毒力基因 

分 类 号：S852.61[农业科学—基础兽医学]