胸膜肺炎放线杆菌分离株血清型的PCR鉴定和Apx毒素基因检测  

Molecular Serotyping and Apx Gene Profile Analysis of Actinobacillus pleuropneumoniae Isolates

作　　者：卢碧凯 袁秀芳[2] 徐丽华[2] 余斌[2] 苏菲[2] 叶十一 陈怡洁 蒋利明 张晖 李军星[2] LU Bikai;YUAN Xiufang;XU Lihua;YU Bin;SU Fei;YE Shiyi;CHEN Yijie;JIANG Liming;ZHANG Hui;LI Junxing(School of Life Science and Technology,Foshan University,Foshan 528200,China;Institute of Animal Husbandry and Veterinary Science,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;Taizhou Animal Disease Prevention and Control Center,Taizhou 317700,China)

机构地区：[1]佛山科学技术学院生命科学与工程学院,佛山528200 [2]浙江省农业科学院畜牧兽医研究所,杭州310021 [3]浙江省台州市动物疫病预防控制中心,台州317700

出　　处：《畜牧兽医学报》2023年第3期1341-1346,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：浙江省自然科学基金(LY21C180002);浙江省重点研发计划(2019C02052-3);台州市农业科技项目。

摘　　要：旨在调查胸膜肺炎放线杆菌流行株的血清型及Apx毒素基因携带情况,采用PCR方法对APP分离株进行血清型鉴定和Apx毒素基因检测。结果表明,64个分离株分别被鉴定为2、5、7、8、12、15、19共7个血清型。7个血清型中发现12种不同的Apx毒力因子模式。毒力因子分析结果显示,同一血清型的不同菌株携带的Apx毒素基因存在差异,不同血清型的菌株也可能会携带相同模式的Apx毒素基因。本研究表明,血清8型胸膜肺炎放线杆菌是浙江省的优势血清型,血清19型菌株在国内首次被检测到。血清型与所携带的Apx毒素基因之间不存在必然联系。The study aimed to investigate the serotypes and Apx toxin genes of Actinobacillus pleuropneumoniae clinical isolates.PCR was used to identify the serotypes and detect the Apx toxin genes of APP isolates.The results showed that 64 isolates were identified as serotypes 2,5,7,8,12,15,19 respectively.A total of 12 different Apx virulence factor patterns were found in 7 serotypes.The results of virulence factor analysis showed that different strains of the same serotype carried different Apx toxin genes,and the strains of different serotypes may also carry the same pattern of Apx toxin genes.A.pleuropneumoniae serotype 8 was the dominant serotype in Zhejiang Province,and serotype 19 was detected for the first time in China.There is no correlation between the Apx toxin gene and the serotype.

关 键 词：胸膜肺炎放线杆菌 血清分型 APX 毒力因子 PCR 

分 类 号：S852.619[农业科学—基础兽医学]