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作 者:高健耘 刘世良 邹胜男 周昊 侯成林[3] 周丽伟 GAO Jianyun;LIU Shiliang;ZOU Shengnan;ZHOU Hao;HOU Chenglin;ZHOU Liwei(College of Life Science,Liaoning University,Shenyang,Liaoning 110036,China;State Key Laboratory of Mycology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;College of Life Sciences,Capital Normal University,Beijing 100048,China)
机构地区:[1]辽宁大学生命科学院,辽宁沈阳110036 [2]中国科学院微生物研究所真菌学国家重点实验室,北京100101 [3]首都师范大学生命科学学院,北京100048
出 处:《食用菌学报》2023年第2期102-110,共9页Acta Edulis Fungi
基 金:生物多样性调查评估项目(2019HJ2096001006)。
摘 要:中华肉球菌(Engleromyces sinensis M.A.Whalley,Khalil,T.Z.Wei,Y.J.Yao&Whalley)是分布于我国西南地区的珍稀药用真菌,具有显著的抗氧化和抗菌活性。由于中华肉球菌模式标本采集年代久远,难以获得DNA条形码序列,不利于该真菌资源的利用和保护。通过对中华肉球菌模式标本及新采集自云南、四川省的标本进行形态学比对,确定新采集的5个标本均为中华肉球菌。指定采集自模式产地云南省玉龙县的一个标本为中华肉球菌的附加模式标本,并对其进行形态描述和显微结构绘图;在此基础上,基于tef-1 a、nrSSU、nrLSU和ITS多基因联合系统发育分析进一步确定中华肉球菌隶属于炭角菌科(Xylariaceae)。通过指定中华肉球菌的附加模式标本,并补充该物种的DNA条形码序列,为其后续开发利用和保护提供基础资料。Engleromyces sinensis is a precious medicinal fungus distributed in southwest China,and has remarkable antioxidant and antibacterial activities.However,the type specimens were collected 65 years ago,thus making it difficult to obtain DNA barcode sequences from them.This drawback is detrimental to the utilization and conservation of this fungal resource.On the basis of morphological examinations among type specimens of E.sinensis and newly sampled specimens from Yunnan and Sichuan,five new collections were identified as E.sinensis.One of them from the type locality Yulong County,Yunnan was designated as an epitype.An illustrated description was provided for the epitype.Phylogenetic analysis based on concatenated tef-1a,nrSSU,nrLSU and ITS sequences further confirmed that E.sinensis belongs to Xylariaceae.From this epitype of E.sinensis,its DNA barcode sequences were supplemented,which provided a basis for the subsequent utilization and conservation of E.sinensis.
分 类 号:S567.3[农业科学—中草药栽培]
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