黄芪多糖在深Ⅱ度烧伤大鼠创面愈合中的作用及其机制  

Effects and mechanism of astragalus polysaccharide on wound healing of deep partial-thickness burns in rats

作　　者：郑凡[1] 蔡玉娥[1] 李黎[1] 韩大伟[2] Zheng Fan;Cai Yu'e;Li Li;Han Dawei(Department of Burns and Plastic Surgery,the First People's Hospital of Shangqiu,Shangqiu 476100,China;Department of Burns,the First People's Hospital of Zhengzhou,Zhengzhou 450004,China)

机构地区：[1]商丘市第一人民医院烧伤整形外科,商丘476100 [2]郑州市第一人民医院烧伤科,郑州450004

出　　处：《中华烧伤与创面修复杂志》2023年第3期256-263,共8页Chinese Journal of Burns And Wounds

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20191002)。

摘　　要：目的探讨黄芪多糖对深Ⅱ度烧伤大鼠创面愈合的影响及其机制。方法采用实验研究方法。取50只雄性7周龄SD大鼠,按照随机数字表法分为正常组、单纯烧伤组、黄芪多糖组、抑制剂组和抑制剂+黄芪多糖组,每组10只。除正常组外,其余4组大鼠均在背部制作1个深Ⅱ度烧伤创面。正常组、单纯烧伤组大鼠腹腔注射生理盐水,其余3组大鼠分别注射黄芪多糖和/或整合素连接激酶(ILK)抑制剂。注射7 d后,计算4组烧伤大鼠创面愈合率,采用酶联免疫吸附测定(ELISA)法检测5组大鼠血清中γ干扰素、白细胞介素2(IL-2)和肿瘤坏死因子α(TNF-α)含量。取正常组大鼠正常皮肤组织及4组烧伤大鼠创面组织,测定水分并计算水分占比,采用ELISA法检测γ干扰素、IL-2和TNF-α的含量,采用实时荧光定量反转录PCR法检测表皮生长因子(EGF)、碱性成纤维细胞生长因子(bFGF)以及ILK mRNA表达量,采用蛋白质印迹法检测ILK、蛋白激酶B(Akt)、磷酸化Akt(p-Akt)、糖原合成激酶-3β(GSK-3β)及磷酸化GSK-3β(p-GSK-3β)蛋白表达量。对数据行单因素方差分析、LSD检验。结果注射7 d后,黄芪多糖组大鼠创面愈合率为(67±5)%,明显高于单纯烧伤组的(52±4)%和抑制剂+黄芪多糖组的(59±5)%(P值均<0.05);抑制剂+黄芪多糖组大鼠创面愈合率明显高于抑制剂组的(48±4)%(P<0.05)。注射7 d后,与正常组大鼠血清或正常皮肤组织比较,单纯烧伤组大鼠血清中γ干扰素、TNF-α、IL-2含量和创面组织水分占比均明显升高(P<0.05);与黄芪多糖组比较,单纯烧伤组和抑制剂+黄芪多糖组大鼠血清中γ干扰素、TNF-α、IL-2含量及创面组织水分占比均明显升高(P<0.05);与抑制剂组比较,抑制剂+黄芪多糖组大鼠血清中γ干扰素、TNF-α、IL-2含量及创面组织水分占比均明显降低(P<0.05)。注射7 d后,与正常组大鼠正常皮肤组织比较,单纯烧伤组大鼠创面组织中γ干扰素、TNF-�Objective To investigate the effects and mechanism of astragalus polysaccharide(APS)on wound healing of deep partial-thickness burns in rats.Methods The experimental study method was used.Fifty 7-week-old male Sprague-Dawley rats were divided into normal group,simple burn group,APS group,inhibitor group,and inhibitor+APS group according to the random number table,with 10 rats in each group.Except for normal group,rats in the other 4 groups were inflicted with a deep partial-thickness burn wound on the back.Rats in normal group and simple burn group were intraperitoneally injected with normal saline,and rats in the other three groups were injected with APS and/or integrin-linked kinase(ILK)inhibitor,respectively.After 7 days of injection,the wound healing rate of rats with burns in the four groups was calculated,and the serum content of interferon-γ,interleukin-2(IL-2),and tumor necrosis factorα(TNF-α)in rats in 5 groups was determined by enzyme-linked immunosorbent assay(ELISA).The normal skin tissue of rats in normal group and wound tissue of rats with burns in the four groups were taken,the water content was determined and the water ratio was calculated,the content of interferon-γ,IL-2,and TNF-αwas detected by ELISA,the mRNA expressions of epidermal growth factor(EGF),basic fibroblast growth factor(bFGF),and ILK were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction,and the protein expressions of ILK,protein kinase B(Akt),phosphorylated Akt(p-Akt),glycogen synthetic kinase-3β(GSK-3β),and phosphorylated GSK-3β(p-GSK-3β)were detected by Western blotting.Data were statistically analyzed with one-way analysis of variance and least significant difference test.Results After 7 days of injection,the wound healing rate of rats in APS group was(67±5)%,which was significantly higher than(52±4)%in simple burn group and(59±5)%in inhibitor+APS group(with all the P values<0.05).The wound healing rate of rats in inhibitor+APS group was significantly higher than(48±4)%i

关 键 词：烧伤 炎症 伤口愈合 黄芪多糖 整合素连接激酶 

分 类 号：R644[医药卫生—外科学] R283[医药卫生—临床医学] R364.5