重庆养殖中华鳖群体遗传多样性的RAPD分析  

RAPD Analysis of Population Genetic Diversity of Pelodiscus sinensis in Chongqing

作　　者：赵华一 刁晓明[2] 李华[1] 杨焕超 陈彦伶[1] 涂全宇 刘钊 ZHAO Hua-yi;DIAO Xiao-ming;LI Hua(Fisheries Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu,Sichuan 611730;College of Fisheries,Southwest University/Chongqing Three Gorges Ecological Fisheries Industry Technology Research Institute,Beibei,Chongqing 400715)

机构地区：[1]四川省农业科学院水产研究所,四川成都611730 [2]西南大学水产学院/重庆三峡生态渔业产业技术研究院,重庆400715

出　　处：《安徽农业科学》2023年第5期67-69,73,共4页Journal of Anhui Agricultural Sciences

基　　金：重庆市农业委员会“生态渔产业体系”项目(2015-40809415);重庆市基础科学与前沿技术研究项目(cstc2015jcyjA0327);重庆市水产科技创新联盟(4322200022)。

摘　　要：[目的]利用RAPD标记技术对重庆4个养殖地区中华鳖的遗传多样性进行分析。[方法]选取(潼南、永川、江津、长寿)4个地区养殖中华鳖样本,运用RAPD方法对4个地区的中华鳖遗传多样性与其遗传距离进行了分析。[结果]用20个随机扩增引物得到142个扩增片段,多态片段数量为95个,4个地区的多态位点比例范围为50.78%~64.54%,总多态位点比例为66.90%,总基因多样性为0.227,种群内基因多样性为0.135,遗传分化系数为0.307,基因流为1.127。4个养殖中华鳖群体的遗传距离为0.049~0.151。[结论]重庆4个地区养殖中华鳖遗传多样性较高,且大部分遗传变异存在于种群内,个体间亲缘关系较近,遗传变异度较小。[Objective]This article uses RAPD marker technology to analyze the genetic diversity of four Pelodiscus sinensis in Chongqing area.[Method]In this study,we mainly selected farmed Pelodiscus sinensis samples from 4 regions(Tongnan,Yongchuan,Jiangjin,and Changshou).[Result]By using 20 random amplification primers,142 amplified fragments were obtained,of which the number of polymorphic fragments was 95.The polymorphism ratio in the four regions ranged from 50.78%to 64.54%,and the total polymorphism ratio was 66.90%.The total genetic diversity of the four populations was 0.227,the genetic diversity within the population was 0.135,the genetic differentiation coefficient was 0.307,and the gene flow data was 1.127.The genetic distance of the four farmed Pelodiscus sinensis populations is between 0.049 and 0.151.[Conclusion]The genetic diversity of farmed Pelodiscus sinensis in the 4 regions of Chongqing is high,and most of the genetic variation exists within the population,and the genetic relationship between individuals is relatively close,and the degree of genetic variation is small.

关 键 词：中华鳖 遗传多样性 RAPD 重庆地区 

分 类 号：S966.5[农业科学—水产养殖]