25份长荚豇豆品种萌芽期耐冷性综合评价  

Cold Tolerance Comprehensive Evaluation of 25 Long-podded Cowpea Cultivars at Germination Stage

作　　者：黄伟康 符启位 郭静依 冯青 贾静静 罗丰 HUANG Weikang;FU Qiwei;GUO Jingyi;FENG Qing;JIA Jingjing;LUO Feng(Institute of Plant Protection,Hainan Academy of Agricultural Sciences(Research Center of Quality Safety and Standards for Agricultural Products of Hainan Academy of Agricultural Sciences)/Key Laboratory of Plant Diseases and Pest Control of Hainan Province/Scientific Observation and Experiment Station of Crop Pests in Haikou,Ministry of Agriculture,Hainan 571100;Sanya Nanfan Research Institute of Hainan University;Sanya Academy of Tropical Agricultural Sciences)

机构地区：[1]海南省农业科学院植物保护研究所(海南省农业科学院农产品质量安全与标准研究中心)/海南省植物病虫害防控重点实验室/农业农村部海口有害生物科学观测实验站,海口571100 [2]海南大学三亚南繁研究院 [3]三亚市热带农业科学研究院

出　　处：《长江蔬菜》2023年第6期40-45,共6页Journal of Changjiang Vegetables

基　　金：海南省重大科技项目(zdkj201901);海南省财政科技计划资助(SQKY2022-004)。

摘　　要：本研究以25份长荚豇豆品种资源为材料,通过测量14℃低温胁迫下与25℃常温下的发芽率、胚根长、胚根质量、总鲜质量,并计算相对值等6个指标,采用相关性分析、主成分分析、隶属函数分析、聚类分析,综合评价长荚豇豆耐冷性。结果表明,低温胁迫对2号和18号品种RGR无影响,对18号品种RGI无影响,对4号和20号品种RTM无影响,但对其他品种的RGR、RGI、RVI、RRL、RRM、RTM均有不同程度的影响,其中,受影响最大的为RGI,其变异系数高达42.07%,其次为RRM,变异系数为36.06%,RTM最小,变异系数为7.44%。利用主成分分析法可将6个单项指标转换为3个相互独立的综合指标。根据权重和隶属函数的分析得到耐冷性综合评价D值,并通过聚类分析可以将25份豇豆品种的耐冷性分为3类,Ⅰ类为耐冷较差型共有8个品种,Ⅱ类为中度耐冷型共有16个品种,Ⅲ类为耐冷型共有1个品种,为18号品种。通过逐步线性回归分析建立豇豆萌芽期耐冷性评价最优回归方程为:D=-1.27+0.004X_(1)+0.01X_(2)+0.01X_(3)+0.005X_(4)+0.04X_(5)+0.002X_(6)。The germination rate,radicle length,radicle weight and total fresh weight of 25 long-podded cowpea cultivars were measured under 14℃low temperature stress and 25℃normal temperature,and 6 indicators such as relative value were calculated.Correlation analysis,principal component analysis,membership function analysis and cluster analysis were used to comprehensively evaluate the cold tolerance of long-podded cowpeas.The results showed that low temperature stress had no effect on RGR of No.2 and No.18 cultivars,no effect on RGI of No.18 cultivar,no effect on RTM of No.4 and No.20 cultivars,but had different effects on RGR,RGI,RVI,RRL,RRM and RTM of other cultivars.Among them,RGI was affected the most,with a coefficient variation of 42.07%,followed by RRM,with a coefficient variation of 36.06%,and RTM was affected the least,with a coefficient variation of 7.44%.Using principal component analysis,6 single indicators could be converted into 3 independent comprehensive indicators.According to the analysis of weight and membership function,the comprehensive evaluation D value of cold tolerance was obtained,and the cold tolerance of 25 cowpea cultivars could be divided into three categories by cluster analysis.There were 8 cultivars in classⅠwith poor cold tolerance,16 cultivars in classⅡwith moderate cold tolerance,and 1 cultivar in classⅢwith cold tolerance,which was No.18 cultivar.The optimal regression equation for cold tolerance evaluation of cowpeas at germination stage was established by stepwise linear regression analysis as follows:D=-1.27+0.004X_(1)+0.01X_(2)+0.01X_(3)+0.005X_(4)+0.04X_(5)+0.002X_(6).

关 键 词：长荚豇豆 耐冷性 萌芽期 

分 类 号：S643.4[农业科学—蔬菜学]