猪粪沼液培养微藻系统中试试验  

Experiment of the pilot-scale microalgal cultivation system with pig manure biogas slurry

作　　者：卞含笑 韩挺 高风正 卢海凤[1,2] 李保明 叶小梅[3,4] BIAN Hanxiao;HAN Ting;GAO Fengzheng;LU Haifeng;LI Baoming;YE Xiaomei(College of Water Resources and Civil Engineering,China Agricultural University,Beijing 100083,China;Key Laboratory of Agricultural Engineering in Structure and Environment,Ministry of Agriculture and Rural Affairs,Beijing 100083,China;Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Key Laboratory for Crop and Animal Integrated Farming of Ministry of Agriculture and Rural Affairs,Nanjing 210014,China;Wageningen University,Bioprocess Engineering,Algae PARC,Wageningen 6700 AA,Netherlands)

机构地区：[1]中国农业大学水利与土木工程学院,北京100083 [2]农业农村部设施农业工程重点实验室,北京100083 [3]江苏农业科学院畜牧研究所,南京210014 [4]农业农村部种养结合重点实验室,南京210014 [5]Wageningen University,Bioprocess Engineering,Algae PARC,Wageningen 6700 AA,Netherlands

出　　处：《农业工程学报》2023年第2期157-165,共9页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国际(地区)合作与交流项目(51861125103);北京市科技计划(Z161100001316009);江苏省农业自主创新资金项目(CX(21)3077)。

摘　　要：为实现猪粪沼液资源化的大规模应用,该研究以小球藻(Chlorella E2E)为试验藻种,开展了中试规模的“室内纯培养+室外纯培养+室外沼液培养”耦合模式多级放大沼液培养微藻试验:考察了各级培养试验中Chlorella E2E的生长状况、沼液中污染物的去除率及微生物多样性,最大培养体积达12.31 m^(3),系统运行时长达274 d。结果表明,Chlorella E2E经过多批次纯培养驯化,在二级柱式反应器内能快速达到最适生长状态,培养至第70天平均生物量可达227.72 mg/L;Chlorella E2E在室外纯培养平板反应器内的产量明显高于室内柱式反应器,培养至第14天生物量可达266.48 mg/L。在采用沼液培养时,户外跑道池中生物量最高达250.02 mg/L,化学需氧量(chemical oxygen demand,COD)、氨氮(ammonia nitrogen,NH_(4)^(+)-N)、总磷(total phosphorus,TP)去除率分别高达40.05%、95.06%、97.52%;跑道池内微生物多样性较高,更易形成菌-藻共生系统,有利于污染物去除。该研究对中试规模多级放大沼液微藻培养系统进行了全面探究,可为户外大规模沼液微藻培养技术的实际应用提供技术参考。A large amount of pig slurry has been generated on the farm each year,particularly with the ever-increasing pig production in recent years.It is a high demand to fully utilize pig manure biogas slurry and microalgae cultivation at a large scale.In this study,a series of experiments were carried out on the multi-stage amplification cultivation with Chlorella E2E using pig manure biogas slurry on a pilot scale,according to the coupled mode of"indoor monoculture+outdoor monoculture+outdoor culture using biogas slurry".The raceway pond reactor was set as the largest area of 12.31 m^(3)in the pilot-scale experiments of"column reactor+plate reactor+runway pool reactor".An investigation was implemented to analyze the growth of Chlorella E2E performance in the pure and biogas slurry cultivation system.A systematic evaluation was also made to clarify the pollutants removal and the diversity of microorganisms in the microalgal cultivation system using pig manure biogas slurry under natural light conditions.Results showed that the Chlorella E2E was gradually adapted to the reactor environment after the multiple batches of pure culture and acclimatization.Specifically,the Chlorella E2E quickly reached the optimum growth state in the second column reactor in indoor monoculture,where the average maximum biomass reached 227.72 mg/L on the 70th day.The fitting relationship was favorable between the inoculum amount,cultivation time and bioaccumulation in each stage of the indoor reactor.A characteristic equation was obtained to predict the biomass yield or cultivation time,particularly for the management of the monoculture mode of Chlorella E2E.The growth capacity of Chlorella E2E in an outdoor monoculture plate reactor was significantly higher than that in an indoor column reactor,where 266.48 mg/L Chlorella E2E biomass was produced on the 14th day.In addition,the highest daily biomass productivity of Chlorella E2E and mean specific growth rate(μ)were achieved in 11.10 mg/L/d and 0.062/d,respectively,in the flate bioreactors un

关 键 词：沼液 反应器 沼液资源化 小球藻 中试放大 多级处理 

分 类 号：X713[环境科学与工程—环境工程]