基于16S rDNA与1H NMR技术探讨高脂血症兔肠道菌群和代谢物变化  

Changes of intestinal flora and metabolites in hyperlipidemia rabbits based on 16S rDNA and~1H NMR technology

作　　者：范丽红[1,2] 黄河 赫飞宇[3] 方园 刘密 常小荣[2] FAN Li-hong;HUANG He;HE Fei-yu;FANG Yuan;LIU Mi;CHANG Xiao-rong(Changsha Social Work College,Changsha,Hu′nan 410004,China;Hu′nan University of Traditional Chinese Medicine,Changsha,Hu′nan 410208,China;不详)

机构地区：[1]长沙民政职业技术学院,湖南长沙410004 [2]湖南中医药大学 [3]厦门大学

出　　处：《中国微生态学杂志》2023年第2期125-131,共7页Chinese Journal of Microecology

基　　金：国家自然科学基金(81874509);国家中医药管理局全国名老中医药专家传承工作室项目(国中医药人教函[2019]41号);常小荣国家级教学名师工作室建设项目;湖南省研究生科研创新项目资助(CX2018B469)。

摘　　要：目的观察高脂血症模型兔的代谢物与肠道微生物变化并分析其相互关系。方法雄性新西兰兔16只,随机分为正常组和模型组,每组8只。正常组喂普通饲料,模型组高胆固醇饮食。12周后比较2组兔血脂水平。利用16S rDNA测序技术检测兔粪便肠道菌群变化,采用1H NMR代谢组学分析结肠代谢物改变。结果与正常组比较,模型组兔血清LDL-C、TG、TC水平显著升高(均P<0.01);模型组兔肠道菌群结构发生改变,厚壁菌门、Lachnospiraceae_NK4A136_group、un_f_Lachnospiraceae、Subdoligranulum等菌群丰度上升,Lachnospiraceae_NK4B4_group、un_o_Gastranaerophilales等菌属丰度下降。2组差异代谢物通路分析主要涉及谷氨酰胺与谷氨酸代谢,丙氨酸、天冬氨酸和谷氨酸代谢,磷脂代谢。Lachnospiraceae_NK4A136_group、un_f_Lachnospiraceae、un_o_Gastranaerophilales等菌属与差异代谢物有较强相关性。结论高脂血症的病理过程可能通过Lachnospiraceae_NK4A136_group、un_f_Lachnospiraceae、Lachnospiraceae_NK4B4_group、un_o_Gastranaerophilales等菌属参与胆碱类代谢,谷氨酰胺与丙氨酸代谢,导致血脂代谢异常。Objective To observe the changes of metabolites and intestinal flora in hyperlipidemia model rabbits and explore their relationship.Methods Sixteen male New Zealand rabbits were randomly divided into normal group and model group with 8 rabbits in each group.The normal group was fed ordinary diet,while the model group was fed high cholesterol diet.The serum lipid levels in the two groups were compared after 12 weeks.The changes of fecal intestinal flora and colonic metabolites were analyzed using 16S rDNA and 1H NMR technique respectively.Results Compared with normal group,the serum LDL-C,TG and TC significantly increased in model group(all P<0.01).The structure of intestinal flora changed,the abundances of Firmicutes,Lachnospiraceae_NK4A136_group,un_f_Lachnospiraceae and Subdoligranulum increased,and those of Lachnospiraceae_NK4B4_group and un_o_Gastranaerophilales decreased in model group.Pathway analysis indicated that the alterations of differential metabolites mainly involved in glutamine and glutamate metabolism,alanine,aspartate and glutamate metabolism and phospholipid metabolism.The abundances of Lachnospiraceae_NK4A136_group,un_f_Lachnospiraceae and un_o_Gastranaerophilales were strongly correlated with differential metabolites.Conclusion The pathological process of hyperlipidemia may participate in choline metabolism,glutamine and alanine metabolism through Lachnospiraceae_NK4A136_group,un_f_Lachnospiraceae,Lachnospiraceae_NK4B4_group and un_o_Gastranaerophilales,resulting in abnormal lipid metabolism.

关 键 词：高脂血症 代谢物 肠道菌群 代谢组学 

分 类 号：R372[医药卫生—病原生物学]