蒙古族药紫花高乌头总黄酮含量测定及其黄酮类化合物的UPLC-Q-Exactive MS定性分析  

Determination of Content of Total Flavonoids in Aconitum septentrionale and UPLC-Q-Exactive MS Qualitative Analysis of the Flavonoids

作　　者：温荣 郭文欣 席琳图雅 斯琴巴特尔 李旻辉[1,2,4,5] WEN Rong;GUO Wen-xing;XILIN Tu-ya;SIQIN Ba-te-er;LI Min-hui(Baotou Medical College,Baotou 014040,China;Inner Mongolia Institute of Traditional Chinese and Mongolian Medicine,Hohhot 010020,China;Xilinguole Meng Mongolian General Hospital,Xilinhot 026000,China;Inner Mongolia Key Laboratory of Characteristic Geoherbs Resources Protection and Utilization,Baotou 014040,China;Inner Mongolia Engineering Research Center of Planting and Development of Astragalus membranaceus var.mongholicus of Geoherbs,Baotou 014040,China)

机构地区：[1]包头医学院,内蒙古包头014040 [2]内蒙古自治区中蒙医药研究院,内蒙古呼和浩特010020 [3]锡林郭勒盟蒙医医院,内蒙古锡林浩特026000 [4]内蒙古自治区特色道地药材资源保护与利用重点实验室,内蒙古包头014040 [5]道地药材蒙古黄芪种植与开发内蒙古自治区工程研究中心,内蒙古包头014040

出　　处：《中国现代中药》2023年第1期38-46,共9页Modern Chinese Medicine

基　　金：锡林郭勒盟科技计划项目(201801)。

摘　　要：目的:比较不同生长年限蒙古族药紫花高乌头栽培品全草中总黄酮的含量,并对黄酮类化合物进行定性分析。方法:通过超声波辅助提取结合紫外-可见分光光度计法测定4种不同生长年限紫花高乌头全草中总黄酮含量,采用超高效液相色谱-静电场轨道阱高分辨质谱法(UPLC-Q-Exactive MS),用Thermo Scientific Hypersil GOLD^(TM)C_(18)色谱柱分离,0.5%乙酸铵水溶液-乙腈为流动相进行梯度洗脱;采用电喷雾离子源(ESI)、全扫描/数据依赖二级扫描(Full MS/dd-MS2)模式采集数据。结果:1年生紫花高乌头中总黄酮的含量较高;从不同生长年限紫花高乌头栽培品中首次鉴定出10个黄酮类化合物。不同生长年限的紫花高乌头中黄酮类化合物的种类有一定差异。结论:该方法简单、可行,能用于不同生长年限紫花高乌头栽培品中总黄酮的含量测定与定性分析。研究结果可为紫花高乌头药效物质基础的进一步研究提供参考。Objective:To compare the content of total flavonoids in the whole plants of cultivated Aconitum septentrionale of different years old and characterize the flavonoids.Methods:The total flavonoid content in whole plants of one-,two-,three-,and four-year-old A.septentrionale was determined by ultrasonic-assisted extraction combined with ultraviolet-visible spectrophotometer.The flavonoids were separated by ultra-performance liquid chromatography coupled with electrostatic field orbit trap high-resolution mass spectrometry(UPLC-Q-Exactive MS).The UPLC-Q-Exactive MS conditions were Thermo Scientific Hypersil GOLD^(TM)C_(18) column,0.5%ammonium acetate in water-acetonitrile as mobile phase for gradient elution,electrospray ionization(ESI),full scan/data-dependent MS/MS(Full MS/dd-MS2).Results:The total flavonoid content reached the highest in one-year-old A.septentrionale.A total of 10 flavonoids were identified for the first time from A.septentrionale of different years old,and the types of flavonoids were different among plants of different years old.Conclusion:The method is simple and feasible,which can quickly determine the content of total flavonoids in cultivated A.septentrionale of different years old and quantitatively analyze the flavonoids.This study can serve as a reference for further research on the medicinal substances.

关 键 词：紫花高乌头 栽培品 黄酮 超高效液相色谱-静电场轨道阱高分辨质谱法 

分 类 号：R284[医药卫生—中药学]