基于秀丽隐杆线虫痴呆模型的络塞维抗阿尔茨海默病药效研究  

Efficacy of Rosavin Against Alzheimer′s Disease:Based on Caenorhabditis elegans(CL4176)Model

作　　者：梁丽娜 高雅婷 陈旭[1] 刘永刚[1] 张园园[1] LIANG Li-na;GAO Ya-ting;CHEN Xu;LIU Yong-gang;ZHANG Yuan-yuan(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《中国现代中药》2023年第1期58-63,共6页Modern Chinese Medicine

摘　　要：目的:基于秀丽隐杆线虫(以下简称线虫)痴呆模型,探究络塞维抗阿尔茨海默病(AD)的作用及可能机制。方法:以转基因线虫CL4176为模型,在显微镜下考察络塞维对线虫瘫痪情况、寿命、运动能力、吞咽频率、活性氧(ROS)水平、抗氧化酶活力等指标的影响。结果:络塞维能够显著降低痴呆模型线虫瘫痪率(P<0.05),延长其寿命,增强其运动能力,显著提高其体内超氧化物歧化酶和过氧化氢酶的活力(P<0.05),显著降低其体内ROS水平和丙二醛含量(P<0.05)。络塞维对模型线虫的吞咽频率没有影响。结论:基于线虫痴呆模型,络塞维有抗阿尔茨海默病的作用,机制可能与改善氧化应激有关。Objective:To explore the anti-Alzheimer′s disease(AD)effect and possible mechanism of rosavin with Caenorhabditis elegans.Methods:Transgenic C.elegans CL4176 with AD was used,and the effect of rosavin on the paralysis,lifespan,mobility,pharyngeal pumping frequency,reactive oxygen species,and activity of antioxidant enzymes of C.elegans CL4176 was observed under the microscope.Results:Rosavin can reduce the paralysis rate(P<0.05),prolong the lifespan,improve the mobility,increase the activity of superoxide dismutase and catalase(P<0.05),and decrease the reactive oxygen species and malondialdehyde content in the C.elegans CL4176(P<0.05).It had no influence on the pharyngeal pumping rate of the nematodes.Conclusion:It is demonstrated that rosavin showed anti-AD effect on C.elegans model,and the possible mechanism may have to do with improving oxidative stress.

关 键 词：络塞维 抗氧化 秀丽隐杆线虫CL4176 阿尔茨海默病 

分 类 号：R285[医药卫生—中药学]