海南黑山羊与其F1代杂交羊皮下脂肪转录组测序比较分析  

Comparative analysis of transcriptome in subcutaneous fat between Hainan black goat and Nubian×Hainan black hybrid goat

作　　者：晁哲[1] 任钰为 孙瑞萍[1] 魏立民[1] 刘海隆[1] 邢漫萍 黄丽丽[1] 郑心力 CHAO Zhe;REN Yuwei;SUN Ruiping;WEI Limin;LIU Hailong;XING Manping;HUANG Lili;ZHENG Xinli(Institute of Animal Science and Veterinary Medicine,Hainan Academy of Agricultural Sciences/Key Laboratory of Tropical Animal Breeding and Disease Research,Haikou 571100,China)

机构地区：[1]海南省农业科学院畜牧兽医研究所/海南省热带动物繁育与疫病研究重点实验室,海南海口571100

出　　处：《畜牧与兽医》2023年第3期1-7,共7页Animal Husbandry & Veterinary Medicine

基　　金：海南省自然科学基金创新研究团队项目(320CXTD445);海南省科协青年科技英才学术创新计划项目(QCXM201802);中央引导地方科技发展专项资金(ZY2022HN09)。

摘　　要：为了研究海南黑山羊与其F1代杂交羊(努比亚山羊公羊与海南黑山羊母羊杂交)皮下脂肪组织的遗传差异,本研究采用RNA-Seq技术和生物信息学方法对2种山羊皮下脂肪组织进行转录组测序分析,运用DESeq鉴定2种山羊皮下脂肪组织间差异表达的mRNAs和lncRNAs,对差异表达的mRNAs进行GO功能、KEGG通路富集分析,同时对差异表达的lncRNAs进行靶基因预测分析。结果显示:海南黑山羊与其F1代杂交羊皮下脂肪间存在330个差异表达的mRNAs,其中4个基因在F1代杂交羊皮下脂肪中特异表达、1个基因在海南黑山羊皮下脂肪中特异表达,在330个差异表达的mRNAs中,根据差异倍数大小,排名前10位的基因分别是PLXDC1、AOC1、SHROOM3、NTS、SPATS2、LOC102181165、BDKRB2、GSDMA、ETNK2、EPOR;存在138个差异表达的lncRNAs,其中9个在F1代杂交羊皮下脂肪中特异表达、5个在海南黑山羊皮下脂肪中特异表达。此外,本研究共获得143个差异表达lncRNAs顺式作用靶基因和135个差异表达lncRNAs反式作用靶基因。本研究为进一步研究山羊皮下脂肪沉积的遗传机理提供科学依据。The aim of this study was to investigate the genetic differences in the subcutaneous adipose tissues between Hainan black goats and Nubian×Hainan black hybrid goats. Here, the RNA-Seq technology and bioinformatics methods were used to perform the transcriptome sequencing and analyses. The differentially expressed mRNAs & lncRNAs in the subcutaneous adipose tissues of Hainan black goats and Nubian×Hainan black hybrid goats were identified by the DESeq software, and the GO function and KEGG enrichment were conducted using the differentially expressed mRNAs. Meanwhile, the target genes of the differentially expressed lncRNAs were predicted. The results showed that 330 mRNAs were differentially expressed in the two goat groups, including 4 mRNAs which were only detected in the subcutaneous fat of the F1 hybrid goats, one mRNA which was only detected in the subcutaneous fat of the Hainan black goats. Among the 330 differentially expressed mRNAs, the abundances ranked in the top 10 differentially expressed mRNAs were PLXDC1, AOC1, SHROOM3, NTS, SPATS2, LOC102181165, BDKRB2, GSDMA, ETNK2 and EPOR, respectively. Moreover, a total of 138 differentially expressed lncRNAs were identified. Among them, 9 lncRNAs were only detected in the F1 hybrid goat, and 5 lncRNAs were only detected in the Hainan black goat. In addition, 143 cis-acting target genes and 135 trans-acting target genes of differentially expressed lncRNAs were obtained. Taken together, these results provided a good foundation for future research on the genetic mechanisms of subcutaneous fat deposition in goats.

关 键 词：海南黑山羊 皮下脂肪 基因 信使RNA 长链非编码RNA 

分 类 号：S827[农业科学—畜牧学]