纳米凝胶提高硫酸头孢喹肟抗菌活性研究  

Enhancement of antibacterial activity of cefquinome sulfate against Staphylococcus aureus small colony variants by nanogels

作　　者：罗万和 周继萍 鞠牧洁 刘金环 买热亚木古力·尼吉米丁 古丽乃再尔·艾麦提 弓超 韩俊成 LUO Wanhe;ZHOU Jiping;JU Mujie;LIU Jinhuan;MAIREYAMUGULI Nijimidin;GULINEZAIR Aimeti;GONG Chao;HAN Juncheng(College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China;College of Animal Science and Technology,Tarim University,Alar 843300,China;Engineering Laboratory of Diagnosis,Prevention and Control of Animal Diseases,Xinjiang Production and Construction Corps,Alar 843300,China;Xinjiang Key Laboratory of Research on and Creation of New Drugs for Herbivores,Urumqi 830052,China)

机构地区：[1]新疆农业大学动物医学学院,新疆乌鲁木齐830052 [2]塔里木大学动物科学与技术学院,新疆阿拉尔843300 [3]新疆生产建设兵团塔里木动物疫病诊断与防控工程实验室,新疆阿拉尔843300 [4]新疆草食动物新药研究与创制重点实验室,新疆乌鲁木齐830052

出　　处：《畜牧与兽医》2023年第3期62-67,共6页Animal Husbandry & Veterinary Medicine

基　　金：新疆维吾尔自治区自然科学基金项目(2019D01B17);新疆农业大学校前期资助课题项目(XJAU201710);塔里木大学校长基金项目(TDZKSS202144);塔里木畜牧科技兵团重点实验室开放课题项目(HS202108)。

摘　　要：为治疗金黄色葡萄球菌小菌落变异体(SCVs)感染,分别以羧甲基纤维素钠和明胶为阴、阳离子载体,通过静电作用制备一种硫酸头孢喹肟纳米凝胶。以溶胀率为指标,筛选最优配方;以外观性状、扫描电镜图、包封率(EE)、载药量(LC)、粒径和Zeta电位为指标,进行质量评价;通过溶血性试验进行安全性评价;采用微量肉汤稀释法评价硫酸头孢喹肟原料药、市售硫酸头孢喹肟注射液和硫酸头孢喹肟纳米凝胶对SCVs的抗菌活性。结果表明,当明胶500 mg、羧甲基纤维素钠200 mg、三聚磷酸钠0.5 mg时为最优配方,为均一淡黄色;扫描电镜图显示硫酸头孢喹肟被明胶、羧甲基纤维素钠和三聚磷酸钠所交联的网络结构所包裹,网络大小均一;平均粒径为(75.72±2.62)nm, Zeta电位为(-0.010 2±0.001 4)mV,EE和LC分别为(85.68±2.41)%和(22.35±1.93)%;且硫酸头孢喹肟纳米凝胶在3 h内均未有溶血现象发生;硫酸头孢喹肟原料药、市售硫酸头孢喹肟注射液和硫酸头孢喹肟纳米凝胶对SCVs的最小抑菌浓度分别为2、1和0.5μg/mL。故本研究所制备的硫酸头孢喹肟纳米凝胶具有较小的纳米尺寸和良好的生物相容性,可为提高由SCVs引起的感染性疾病疗效提供依据。In order to treat Staphylococcus aureus small colony variants(SCVs) infection, cefquinome sulfate nanogel was prepared by electrostatic interaction with sodium carboxymethyl cellulose and gelatin as cation and anion carriers, respectively. The optimum formula was selected with the swelling rate as the index;the appearance, scanning electron microscopy, encapsulation efficiency(EE), drug loading(LC), particle size and zeta potential were used as indexes for evaluation;the safety was evaluated by hemolysis test;and the antibacterial activity of cefquinome sulfate, cefquinome sulfate injection and cefquinome sulfate nanogel against SCVs were evaluated by broth microdilution. The results showed that the best formula was 500 mg gelatin, 200 mg sodium carboxymethyl cellulose, and 0.5 mg sodium tripolyphosphate, in uniformly light yellow. images scanned by electron microscopy showed that cefquinome sulfate was encapsulated by gelatin, sodium carboxymethyl cellulose and sodium tripolyphosphate, and the network size was uniform. The average particle size was(75.72±2.62) nm,zeta potential was(-0.010 2±0.001 4) mV;and EE and LC were(85.68±2.41%) and(22.35±1.93%), respectively. There was no hemolysis within 3 h;and the minimum inhibitory concentrations of cefquinome sulfate, cefquinome sulfate injection and cefquinome sulfate nanogel against SCVs were 2, 1 and 0.5 μg/mL, respectively. Therefore, the cefquinome sulfate nanogel prepared in this study had small nano size and good biocompatibility, which provided a basis for improving the efficacy of treating infectious diseases caused by SCVs.

关 键 词：纳米凝胶 小菌落变异体 硫酸头孢喹肟 抗菌活性 制备 

分 类 号：S859.5[农业科学—临床兽医学]