Effect of arsenite on the proteome of earthworms Eisenia fetida  

作　　者：Yali Wang Yinsheng Li Hongpei Geng Qian Zuo Michelle Thunders Jiangping Qiu 

机构地区：[1]School of Environmental Engineering,Henan University of Technology,Zhengzhou 450001,China [2]School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China [3]State Environmental Protection Key Laboratory of Monitoring for Heavy Metal Pollutants,Changsha 410000,China [4]Department of Pathology and Molecular Medicine,University of Otago,Wellington 6242,New Zealand

出　　处：《Soil Ecology Letters》2023年第1期181-194,共14页土壤生态学快报（英文）

基　　金：supported by the Shanghai Agriculture Applied Technology Development Program of China(2021 No.2-2);the Natural Science Projects of Henan University of Technology,China(No.2019BS037);the Open Fund Project of State Environmental Protection Key Laboratory of Monitoring for Heavy Metal Pollutants(SKLMHM202106);the National Natural Science Foundation of China(No.41471203).

摘　　要：Arsenic(As)is broadly distributed due to natural and anthropogenic sources,and it is toxic to organisms.This study aimed to investigate the proteomic response in earthworm exposed to As^(3+).Earthworms were exposed to As^(3+)in soil at 5-80 mg kg1,and samples were collected after 60 days exposure.Two-dimensional electrophoresis(2-DE)was used to separate the proteins in earthworm homogenate,then differentially expressed proteins(DEPs)were identified using MALDI-TOF/TOF-MS analysis.After 2-DE,36 DEPs were found and 24 of them were successfully identified.79.2%of DEPs were upregulated compared to the control group.The maximum fold change reached 53.8 in spot 3108 in the 80 mg kg^(-1)As group.Two proteins were not found in the control group but found in the As treated groups.Proteins were grouped into metabolism,signal transduction,stress-related,transport,regulation,and predicted/hypothetical protein categories based on their function.The protein-protein interaction between the DEPs indicated that serum albumin(ALB)is very important,related to 6 other proteins.Proteins were then verified by western blot,the results were in agreement with the proteomic analyses.The identification of induced or repressed proteins because of As^(3+)in earthworms is helpful to explore the underlying mechanisms of soil arsenic ecotoxicity.

关 键 词：EARTHWORM As^(3+) Two dimensional electrophoresis MALDI-TOF/TOF-MS Proteomics 

分 类 号：X53[环境科学与工程—环境工程] S154.1[农业科学—土壤学]