Newly Detected Transmission of bla_(KPC-2) by Outer Membrane Vesicles in Klebsiella Pneumoniae  

作　　者：Liu-jun CHEN Xiao-peng JING Dong-li MENG Ting-ting WU Huan ZHOU Rui-ling SUN Xiao-chun MIN Rong LIU Ji ZENG 

机构地区：[1]Department of Clinical Laboratory,Wuhan Fourth Hospital,Wuhan,430030,China [2]Key Laboratory of Ministry of Education for Neurological Disorders,Department of Pathophysiology,School of Basic Medicine,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430030,China

出　　处：《Current Medical Science》2023年第1期80-85,共6页当代医学科学（英文）

基　　金：supported by the National Natural Science Foundation of China(No.31771189);the Wuhan Health Commission(No.WX18C17 and No.WX19Q31);the Natural Science Foundation of Hubei Province,China(No.2017CFA065 and No.WJ2019H378).

摘　　要：Objective The prevalence of carbapenem-resistant Klebsiella pneumoniae(CR-KP)is a global public health problem.It is mainly caused by the plasmid-carried carbapenemase gene.Outer membrane vesicles(OMVs)contain toxins and other factors involved in various biological processes,includingβ-lactamase and antibiotic-resistance genes.This study aimed to reveal the transmission mechanism of OMV-mediated drug resistance of Klebsiella(K.)pneumoniae.Methods We selected CR-KP producing K.pneumoniae carbapenemase-2(KPC-2)to study whether they can transfer resistance genes through OMVs.The OMVs of CR-KP were obtained by ultracentrifugation,and incubated with carbapenem-sensitive K.pneumoniae for 4 h.Finally,the carbapenem-sensitive K.pneumoniae was tested for the presence of bla_(KPC-2)resistance gene and its sensitivity to carbapenem antibiotics.Results The existence of OMVs was observed by the electron microscopy.The extracted OMVs had bla_(KPC-2)resistance gene.After incubation with OMVs,bla_(KPC-2)resistance gene was detected in sensitive K.pneumoniae,and it became resistant to imipenem and meropenem.Conclusion This study demonstrated that OMVs isolated from KPC-2-producing CR-KP could deliver bla_(KPC-2)to sensitive K.pneumoniae,allowing the bacteria to produce carbapenemase,which may provide a novel target for innovative therapies in combination with conventional antibiotics for treating carbapenem-resistant Enterobacteriaceae.

关 键 词：bla_(KPC-2) carbapenem-resistant Klebsiella pneumoniae CARBAPENEMASE outer membrane vesicles simplified carbapenem inactivation method 

分 类 号：R563.1[医药卫生—呼吸系统]