单细胞组学技术在肾脏发育及损伤机制研究中的应用  

Application of single cell omics technologies in kidney development and injury mechanism research

作　　者：蒋含路(综述) 戴春笋(审校)[1] JIANG Hanlu;DAI Chunsun(Center for Kidney Disease,The Second Affiliated Hospital,Nanjing Medical University,Nanjing 210003,China)

机构地区：[1]南京医科大学第二附属医院肾脏病医学中心,南京210003

出　　处：《肾脏病与透析肾移植杂志》2023年第1期64-68,共5页Chinese Journal of Nephrology,Dialysis & Transplantation

基　　金：江苏省社会发展重点项目(BE2020725)。

摘　　要：近年来,单细胞组学技术的迅速发展促成了肾脏领域的许多重大发现。研究者通过分析单细胞水平产生的数据,能够发现新的细胞类型、精确了解细胞状态及其基因表达模式的变化、分析不同类型细胞间的相互作用,从而明确疾病的发病机制和研判病变的进展状态。在这篇综述中,我们首先总结分析了当前常用单细胞组学技术的种类和各自的优缺点;进而阐述了单细胞组学技术在揭示肾脏病理生理机制中的应用和主要发现;最后,我们对单细胞组学技术在肾脏疾病的机制研究和临床治疗方面的应用前景进行了初步探讨。During the past decade,rapidly expanding high-throughput single cell omics technologies facilitated a lot of considerable breakthroughs in the field of kidney research. Based on the huge amounts of data generated by single cell omics technology,researchers may discover new cell types,identify the dynamics of cell state and gene expression patterns,explore the cross-talking between different cell types and deeply understand the pathogenesis and progression of diseases. In this review,we summarized the types of current single-cell omics approach,analyzed the benefits and drawbacks of each important omics technology,and probed into the applications and findings of single cell omics in the field of renal pathophysiology. In the end,we also prospected the application of single cell omics in experimental study and targeted therapy of kidney diseases in the future.

关 键 词：单细胞组学 肾脏 发育 损伤 

分 类 号：R692[医药卫生—泌尿科学]