A universal probe for simultaneous detection of six pospiviroids and natural infection of potato spindle tuber viroid(PSTVd) in tomato in China  

作　　者：ZHANG Yu-hong LI Zhi-xin DU Ya-jie LI Shi-fang ZHANG Zhi-xiang 

机构地区：[1]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China [2]Keshan Branch of Heilongjiang Academy of Agricultural Sciences,Qiqihar 161005,P.R.China [3]College of Agronomy and Biotechnology,Hebei Normal University of Science&Technology,Qinhuangdao 066004,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第3期790-798,共9页农业科学学报（英文版）

基　　金：funded by the National Natural Science Foundation of China(31670149)。

摘　　要：Several viroids in the genus Pospiviroid can infect tomato(Solanum lycopersicum)and cause severe diseases,posing a serious threat to tomato production.For simultaneous detection of six tomato-infecting pospiviroids-columnea latent viroid(CLVd),pepper chat fruit viroid(PCFVd),potato spindle tuber viroid(PSTVd),tomato apical stunt viroid(TASVd),tomato chlorotic dwarf viroid(TCDVd),and tomato planta macho viroid(TPMVd),we developed a universal probe based on a highly conserved 61 nt long sequence shared among them.Compared with their specific probes,the universal probe has a similar,though slightly reduced,detection sensitivity and has the advantages of simple and cost-effective preparation and simultaneous detection of the six pospiviroids.In addition,the universal probe was used in dot-blot hybridization assays for a large-scale survey of viroid(s)in tomato plantings in China.Only PSTVd was detected in a few greenhouse-planted tomato plants.Sequence analysis revealed that these tomato PSTVd isolates may have been introduced from tomato seeds imported from abroad.

关 键 词：TOMATO VIROID simultaneous detection molecular hybridization pospiviroids 

分 类 号：S532[农业科学—作物学]